Williams P G, Helmer M A, Wright L C, Dyne M, Fox R M, Holmes K T, May G L, Mountford C E
FEBS Lett. 1985 Nov 11;192(1):159-64. doi: 10.1016/0014-5793(85)80064-8.
Human blood lipoproteins have been characterised by 1H NMR methods and chemical analysis, and comparisons made with the properties of the triglyceride-rich plasma membrane domain found in cancer cells. By means of selective and non-selective T1 experiments, the lipids in HDL and LDL are shown to be in diffusive exchange. In contrast, the lipids of chylomicra and VLDL do not exhibit lipid diffusion, and therefore resemble the neutral lipids of cancer cell plasma membranes. 2D scalar correlated NMR (COSY) spectra of cancer cells or solid tumours are similar to those obtained from VLDL and LDL. The long T2 relaxation value observed for neutral lipid methylenes in metastatic cancer cells (greater than 300 ms) was not observed for any of the 4 lipoproteins studied. None of the lipoprotein classes gave a T2 longer than 250 ms.
人类血液脂蛋白已通过1H NMR方法和化学分析进行了表征，并与癌细胞中富含甘油三酯的质膜结构域的特性进行了比较。通过选择性和非选择性T1实验，发现高密度脂蛋白（HDL）和低密度脂蛋白（LDL）中的脂质存在扩散交换。相比之下，乳糜微粒和极低密度脂蛋白（VLDL）的脂质不表现出脂质扩散，因此类似于癌细胞质膜中的中性脂质。癌细胞或实体瘤的二维标量相关NMR（COSY）光谱与从VLDL和LDL获得的光谱相似。在转移性癌细胞中观察到的中性脂质亚甲基的长T2弛豫值（大于300毫秒）在所研究的4种脂蛋白中均未观察到。没有一种脂蛋白类别给出的T2长于250毫秒。
