Matos Julyana Sthéfanie Simões, Demoliner Meriane, Gularte Juliana Schons, Filippi Micheli, de Abreu Góes Pereira Vyctoria Malayhka, de Barros Marcelo Pereira, Spilki Fernando Rosado
Universidade Feevale, Campus II, ERS-239, N. 2755, Novo Hamburgo, RS, 93525-075, Brazil.
Ecohealth. 2025 Jun 22. doi: 10.1007/s10393-025-01725-x.
This study focuses on monitoring of SARS-CoV-2 in free-living animals in the Vale dos Sinos region, Rio Grande do Sul, Brazil, aiming to verify the presence of this emerging virus using next-generation sequencing (NGS) technique. Rectal and oral swab samples were collected from 52 white-eared opossums (Didelphis albiventris) and submitted to metagenomics and subsequently directed SARS-CoV-2 genome sequencing. Five positive samples were found to originate from these animals' contact with human waste or contaminated water bodies, indicating the spillover of SARS-CoV-2 to D. albiventris.
本研究聚焦于对巴西南里奥格兰德州锡诺斯河谷地区野生动物体内的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）进行监测，旨在利用下一代测序（NGS）技术核查这种新出现病毒的存在情况。从52只白耳负鼠（Didelphis albiventris）采集了直肠和口腔拭子样本，并进行宏基因组学分析，随后进行SARS-CoV-2基因组测序。发现5个阳性样本源于这些动物接触人类粪便或受污染水体，这表明SARS-CoV-2已传播至白耳负鼠。
