Richins Travis, Sapp Sarah G H, Juhasz Alexandra, Cunningham Lucas J, La Course E James, Stothard J Russell, Barratt Joel L N
Division of Parasitic Diseases and Malaria, Laboratory Sciences and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK.
Parasitology. 2025 Jun;152(7):735-744. doi: 10.1017/S0031182025100243.
Following the recent report of strongyloidiasis caused by within a semi-captive colony of baboons in a UK safari park, we investigated the genetic relationships of this isolate with other isolates across the world. Whole-genome sequencing data were generated with later phylogenetic analysis of mitochondrial (mt) cytochrome oxidase subunit 1 () and nuclear ribosomal 18S sequences against 300 published reference genotypes. The putative African origin of the UK was confirmed and full-length mt genome sequences were assembled to facilitate a more detailed phylogenetic analysis of 14 mt coding regions against all available species. Our analyses demonstrated that the UK isolate represented a novel African lineage not previously described. Additional complete mt genomes were assembled for several individual UK safari park worms to reveal a slightly altered mt genome gene arrangement, allowing clear separation from Asian . Furthermore, these UK worms possessed expanded intergenic regions of unknown function that increase their mt genome size to approximately 24 kilobases (kb) as compared with some 16 kb for Asian ; this may have arisen from unique populational founder and genetic drift effects set within the peculiar mixed species baboon and drill ancestry of this semi-captive primate colony. A maximum likelihood phylogeny constructed from 14 mt coding regions also supported an evolutionary distinction between Asian and African .
继英国一个野生动物园半圈养狒狒群体中出现由粪类圆线虫引起的类圆线虫病的近期报告之后，我们调查了该分离株与世界各地其他粪类圆线虫分离株之间的遗传关系。利用线粒体（mt）细胞色素氧化酶亚基1（COI）和核糖体18S序列对300个已发表的粪类圆线虫参考基因型进行系统发育分析，生成了全基因组测序数据。英国粪类圆线虫的假定非洲起源得到证实，并组装了全长mt基因组序列，以便对14个mt编码区域与所有可用的粪类圆线虫物种进行更详细的系统发育分析。我们的分析表明，英国分离株代表了一个以前未描述过的新的非洲谱系。为英国野生动物园的几条个体线虫组装了额外的完整mt基因组，以揭示mt基因组基因排列略有改变，从而能够与亚洲粪类圆线虫明显区分开来。此外，这些英国线虫具有功能未知的扩展基因间隔区，使其mt基因组大小增加到约24千碱基（kb），而亚洲粪类圆线虫约为16 kb；这可能是由于这个半圈养灵长类动物群体中独特的混合物种狒狒和鬼狒血统所产生的独特种群奠基者效应和遗传漂变效应导致的。根据14个mt编码区域构建的最大似然系统发育也支持亚洲和非洲粪类圆线虫之间的进化差异。
