Zhao Huan, Haidamak Juciliane, Noskova Eva, Ilik Vladislav, Pafčo Barbora, Ford Rebecca, Masiria Geraldine, Maure Tobias, Kotale Nichola, Pomat William, Gordon Catherine, Navarro Severine, Horwood Paul F, Constantinoiu Constantin, Greenhill Andrew R, Bradbury Richard S
Emerg Infect Dis. 2025 Sep;31(9):1793-1801. doi: 10.3201/eid3109.241923.
The human-infecting parasite Strongyloides fuelleborni subspecies kellyi has been reported from the island of New Guinea. We analyzed fecal DNA extracts (n = 164) from 19 infants in Papua New Guinea by using Strongyloides real-time PCR and undertook metabarcoding of cox1 and 18S rRNA hypervariable regions I and IV loci. Eight infants were infected with Strongyloides spp.; 7 were infected with S. fuelleborni subsp. fuelleborni and 1 with a Strongyloides sp. previously misattributed to S. fuelleborni subsp. kellyi. Phylogenetic and haplotyping analyses indicated S. fuelleborni in Papua New Guinea belongs to the Indochina subclade of S. fuelleborni subsp. fuelleborni and is not a unique subspecies. We report molecular evidence of S. fuelleborni subsp. fuelleborni infection in humans in the Pacific. Our findings also demonstrate the potential co-existence of an undescribed human-infecting Strongyloides sp. on the island of New Guinea, indicating a need for renewed clinical and epidemiologic investigations into infant strongyloidiasis.
已报道在新几内亚岛存在感染人类的寄生虫——威氏类圆线虫凯利亚种。我们运用类圆线虫实时聚合酶链反应（PCR），对来自巴布亚新几内亚19名婴儿的粪便DNA提取物（n = 164）进行了分析，并对细胞色素氧化酶亚基I（cox1）以及18S核糖体RNA（rRNA）高变区I和IV位点进行了宏条形码分析。8名婴儿感染了类圆线虫属；7名感染了威氏类圆线虫威氏亚种，1名感染了一种先前被错误归类为威氏类圆线虫凯利亚种的类圆线虫。系统发育和单倍型分析表明，巴布亚新几内亚的威氏类圆线虫属于威氏类圆线虫威氏亚种的印度支那亚分支，并非一个独特的亚种。我们报告了威氏类圆线虫威氏亚种在太平洋地区人类中感染的分子证据。我们的研究结果还表明，新几内亚岛可能存在一种未描述的感染人类的类圆线虫，这表明有必要重新开展针对婴儿类圆线虫病的临床和流行病学调查。
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