家蚕卵巢中雌二醇的鉴定。
Identification of estradiol in the ovaries of the silkworm, Bombyx mori.
作者信息
Ohnishi E, Ogiso M, Wakabayashi K, Fujimoto Y, Ikekawa N
出版信息
Gen Comp Endocrinol. 1985 Oct;60(1):35-8. doi: 10.1016/0016-6480(85)90289-8.
PMID:4054586
Abstract
Estradiol was extracted and partially purified from the ovaries of the silkworm, Bombyx mori. Identification of estradiol was done by use of radioimmunoassay (RIA) and by gas chromatography-mass spectrometry (GC-MS) after derivatization into the ethyldimethylsilyl derivative. Concentration of estradiol in the ovaries was estimated to be 176 pg/g (RIA) and 63 pg/g (GC-MS).
摘要
从家蚕（Bombyx mori）的卵巢中提取并部分纯化了雌二醇。通过放射免疫分析（RIA）以及将其衍生化为乙基二甲基硅烷基衍生物后利用气相色谱 - 质谱联用仪（GC-MS）对雌二醇进行了鉴定。卵巢中雌二醇的浓度估计为176 pg/g（RIA法）和63 pg/g（GC-MS法）。
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