Ohnishi E, Mizuno T, Chatani F, Ikekawa N, Sakurai S
Science. 1977 Jul 1;197(4298):66-7. doi: 10.1126/science.867050.
From ovaries dissected from developing and emerged adults of the silkworm, Bombyx mori, two substances having high molting hormone activity were isolated. One of these was identified as 2-deoxy-alpha-ecdysone by means of high-pressure liquid chromatography and mass spectrometry. Although this compound had previously been isolated from the fern Blechnum minus and postulated to be the precursor of alpha-ecdysone, it had not been obtained from insect material. The compound is also contained in the form of a conjugate in ovaries as well as in diapausing silkworm embryos.
从发育中和羽化后的家蚕成虫解剖得到的卵巢中,分离出了两种具有高蜕皮激素活性的物质。其中一种通过高压液相色谱和质谱法被鉴定为2-脱氧-α-蜕皮激素。尽管该化合物此前已从小叶乌毛蕨中分离出来,并被推测为α-蜕皮激素的前体,但尚未从昆虫材料中获得。该化合物在卵巢以及滞育家蚕胚胎中也以共轭物的形式存在。