Grismer L Lee, Kaatz Amanda, Grismer Jesse L, Nguyen Eddie, Grergory Jeren J, Wood Perry L, Murdoch Matthew L, Anuar Shahrul, Onn Chan Kin, Muin Muhamad A, Pawangkhanant Parinya, Suwannapoom Chatmongkon, Poyarkov Nikolay A, Quah Evan S H
Herpetology Laboratory, Department of Biology, La Sierra University, 4500 Riverwalk Parkway, Riverside, California 92505, USA La Sierra University Riverside United States of America.
Department of Herpetology, San Diego Natural History Museum, PO Box 121390, San Diego, California, 92112, USA Universiti Malaysia Sabah Kota Kinabalu Malaysia.
Zookeys. 2025 Jun 12;1241:105-137. doi: 10.3897/zookeys.1241.149552. eCollection 2025.
Phylogenetic analyses based on 1459 base pairs of the mitochondrial gene ND2 and its flanking tRNAs indicate that from the type locality in Sarawak, East Malaysia (Borneo) and from Peninsular Malaysia are not conspecific. Both populations as well as from East Malaysia form a strongly supported monophyletic group even though their relationships to one another remain unresolved. from peninsular Malaysia is described herein as the new species whose type locality is Gunung Belumut, Johor State. is diagnosable from all other species in the group by having statistically different morphospatial positions in multiple factor analyses (MFA) based on size-corrected morphometric and meristic characters. ANOVA analyses of these characters recovered significantly different mean values between and varying combinations of all other group species across several size-corrected morphometric and meristic characters. Genetic variation within is geographically structured across six well supported monophyletic mitochondrial lineages bearing an uncorrected pairwise sequence divergence ranging from 0.97-4.5%. Despite its well supported phylogeographic structure, PCAs and ANOVAs recovered statistically weak morphological separation among the lineages and as such, all are considered conspecific pending a genomic analysis. The phylogeographic structure within the forest-dwelling is quite similar to that of the stream-adapted ranid frog genus and less so to that of the microhabitat specialists of the group and the forest generalist , all of whom are sympatric across Peninsular Malaysia.
基于线粒体基因ND2及其侧翼tRNA的1459个碱基对进行的系统发育分析表明,来自东马来西亚沙捞越(婆罗洲)的模式产地以及来自马来西亚半岛的个体并非同种。尽管这两个种群之间以及与来自东马来西亚的个体之间的关系仍未明确,但它们共同构成了一个得到有力支持的单系类群。本文将来自马来西亚半岛的个体描述为新物种 ,其模式产地为柔佛州的武吉贝卢穆山。通过基于尺寸校正的形态测量和分节特征的多因素分析(MFA), 在形态空间位置上具有统计学差异,从而可与该类群中的所有其他物种区分开来。对这些特征进行方差分析(ANOVA)发现,在多个尺寸校正的形态测量和分节特征方面, 与该类群所有其他物种的不同组合之间的平均值存在显著差异。 内部的遗传变异在地理上呈现出六个得到有力支持的单系线粒体谱系结构,其未校正的成对序列差异范围为0.97%-4.5%。尽管其地理谱系结构得到了有力支持,但主成分分析(PCA)和方差分析在谱系之间发现了统计学上较弱的形态分离,因此,在进行基因组分析之前,所有谱系都被视为同种。栖息于森林中的 的地理谱系结构与适应溪流的林蛙属 的结构非常相似,但与该类群的微生境 specialists和森林泛化种 的结构相似性较低,后两者在马来西亚半岛均同域分布。