使用超声激活和二极管激光疗法相结合的方法评估根管消毒效果。
evaluation of root canal disinfection using a combination of ultrasonic activation and diode laser therapy.
作者信息
Bansode Sonali H, Chole Dayanand G, Bakle Srinivas S, Hatte Nikhil R, Gandhi Neha P, Inamdar Mahenaz R
机构信息
Department of Conservative Dentistry and Endodontics, Pandit Deendayal Upadhyay Dental College, Solapur, Maharashtra, India.
出版信息
J Conserv Dent Endod. 2025 Jun;28(6):510-514. doi: 10.4103/JCDE.JCDE_158_25. Epub 2025 Jun 2.
AIM
evaluation of root canal disinfection using a combination of ultrasonic activation and diode laser therapy.
OBJECTIVE
The primary aim of this study was to evaluate the effectiveness of ultrasonic activation of sodium hypochlorite and diode laser irradiation in enhancing root canal disinfection by reducing bacterial load.
MATERIALS AND METHODS
Thirty patients aged 20-60 years with necrotic lower premolars and periapical infections were selected. After access opening, initial microbial samples (S1) were collected. The canals were then instrumented using ProTaper Next rotary files and irrigated with 2.5% sodium hypochlorite, which was activated ultrasonically using EndoX for 1 min. The second microbial sample (S2) was collected. Subsequently, 980 nm diode laser irradiation was applied in a pulsed mode, and the final microbial sample (S3) was obtained. All the samples were cultured in Brain Heart Infusion Agar (BHI) agar, and colony-forming units (CFU/ml) were determined. Statistical analysis was performed using the SPSS software with a significance threshold of ≤ 0.05.
RESULTS
The mean CFU/ml count significantly reduced from S1 (22.93) to S2 (6.03) after ultrasonic activation and further decreased to S3 (1.04) following diode laser application. The reduction was statistically significant ( < 0.001).
CONCLUSION
The combination of ultrasonic activation of sodium hypochlorite and diode laser irradiation significantly enhances root canal disinfection. Ultrasonic activation improves irrigant penetration, while diode laser provides deeper antimicrobial action.
目的
评估超声激活与二极管激光治疗联合用于根管消毒的效果。
目标
本研究的主要目的是评估次氯酸钠超声激活和二极管激光照射通过降低细菌载量来增强根管消毒的有效性。
材料与方法
选取30例年龄在20 - 60岁之间患有坏死性下颌前磨牙及根尖周感染的患者。开髓后，采集初始微生物样本（S1）。然后使用ProTaper Next旋转锉对根管进行预备，并用2.5%的次氯酸钠冲洗，使用EndoX超声激活1分钟。采集第二个微生物样本（S2）。随后，以脉冲模式应用980 nm二极管激光照射，获得最终微生物样本（S3）。所有样本均在脑心浸液琼脂（BHI）培养基中培养，并测定菌落形成单位（CFU/ml）。使用SPSS软件进行统计分析，显著性阈值≤0.05。
结果
超声激活后，平均CFU/ml计数从S1（22.93）显著降至S2（6.03），在应用二极管激光后进一步降至S3（1.04）。这种降低具有统计学意义（<0.001）。
结论
次氯酸钠超声激活与二极管激光照射联合应用可显著增强根管消毒效果。超声激活可改善冲洗液的渗透，而二极管激光则提供更深层次的抗菌作用。
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