Mishra Ankur, Koul Monika, Abdullah Ahsan, Khan Nausheen, Dhawan Preeti, Bhat Anu
Department of Pedodontics and Preventive Dentistry, Career Post Graduate Institute of Dental Sciences & Hospital, Lucknow, Uttar Pradesh, India.
Department of Pedodontics and Preventive Dentistry, BRS Dental College and Hospital, Panchkula, Haryana, India.
Int J Clin Pediatr Dent. 2022 Sep-Oct;15(5):579-583. doi: 10.5005/jp-journals-10005-2444.
The success of endodontic treatment is achieved by the complete elimination of pulpal infection and protection from future invasion of microorganisms. Due to the complex structure of the root canal, complete eradication of microorganisms is not possible and is a major challenge for successful endodontic treatment. Therefore, microbiological studies are needed to explore the effect of various disinfection methods.
The aim of this study is to compare the effectiveness of root canal disinfection by using a diode laser (in pulse and continuous modes) and sodium hypochlorite by microbiological assessment.
Forty-five patients were selected and randomly divided into three groups. After gaining patency to the root canal the first sample from the root canal was taken using a sterile absorbent paper point and transferred to a sterile tube containing a normal saline medium. Biomechanical preparation was performed with Dentsply Protaper hand files of each corresponding group and was disinfected with group I (diode light amplification by stimulated emission of radiation (LASER) 980 nm with 3 W in continuous mode for 20 seconds), group II (diode LASER 980 nm with 3 W in pulse mode for 20 seconds), group III (irrigated with 5.25% of sodium hypochlorite for 5 minutes). Pre- and post-samples of each group were inoculated on sheep blood agar and examined for any bacterial growth. After the microbial evaluation of the total microbial count of pre- and post-samples, the data obtained were tabulated and statistically analyzed.
The data were evaluated and analyzed using analysis of variance (ANOVA) on Statistical Package for the Social Sciences (SPSS) software. Groups I, II, and III all three groups showed significant differences ( < 0.01) and an overall reduction in the microbial count at postbiomechanical preparation (BMP) as compared to pre-BMP with the highest being in laser in continuous mode (group I) (91.9%), followed by 5.25% sodium hypochlorite (group III) (86.5%) and LASER in pulse mode (group II) (72.0%) the least.
The study concluded that the diode laser in continuous mode is more efficacious than the diode laser in pulse mode and 5.2% sodium hypochlorite, respectively.
Mishra A, Koul M, Abdullah A, Comparative Evaluation of Antimicrobial Efficacy of Diode Laser (Continuous Mode), Diode Laser (Pulse Mode), and 5.25% of Sodium Hypochlorite in Disinfection of Root Canal: A Short Study. Int J Clin Pediatr Dent 2022;15(5):579-583.
牙髓治疗的成功是通过彻底消除牙髓感染并防止微生物未来的侵入来实现的。由于根管结构复杂，不可能完全根除微生物，这是牙髓治疗成功的一项重大挑战。因此，需要进行微生物学研究以探索各种消毒方法的效果。
本研究的目的是通过微生物学评估比较使用二极管激光（脉冲和连续模式）和次氯酸钠进行根管消毒的效果。
选择45例患者并随机分为三组。在打通根管通路后，使用无菌吸水纸尖从根管中采集第一个样本，并转移至含有生理盐水培养基的无菌试管中。各相应组使用登士柏Protaper手动锉进行生物力学预备，然后分别用以下方法消毒：第一组（波长980nm、功率3W的二极管激光连续模式照射20秒），第二组（波长980nm、功率3W的二极管激光脉冲模式照射20秒），第三组（用5.25%的次氯酸钠冲洗5分钟）。每组的术前和术后样本接种于羊血琼脂上，检查是否有细菌生长。在对术前和术后样本的总微生物计数进行微生物评估后，将获得的数据制成表格并进行统计分析。
使用社会科学统计软件包（SPSS）中的方差分析（ANOVA）对数据进行评估和分析。第一组、第二组和第三组均显示出显著差异（P<0.01），并且与生物力学预备前相比，生物力学预备后（BMP）的微生物计数总体减少，其中连续模式激光组（第一组）最高（91.9%），其次是5.25%次氯酸钠组（第三组）（86.5%），脉冲模式激光组（第二组）最少（72.0%）。
该研究得出结论，连续模式的二极管激光分别比脉冲模式的二极管激光和5.2%次氯酸钠更有效。
Mishra A, Koul M, Abdullah A, 根管消毒中二极管激光（连续模式）、二极管激光（脉冲模式）和5.25%次氯酸钠抗菌效果的比较评价：一项简短研究。《国际临床儿科牙科学杂志》2022；15（5）：579 - 583。
