Silva França Álefe Roger, da Silva Batista Beatriz, Silva Diniz Filho Joel Félix, Viana Sousa Rosa Maria, de Menezes Alan Silva, Dos Santos Clenilton Costa, Santos-Oliveira Ralph, Noronha Souza Pedro Filho, da Silva Luzeli Moreira, Rebêlo Alencar Luciana Magalhães
Center for Social Sciences, Health and Technology, Federal University of Maranhão, Advanced Unit, Imperatriz, MA 65900-410, Brazil.
Department of Physics, Laboratory of Biophysics and Nanosystems, Federal University of Maranhão, Campus Bacanga, São Luís, MA 65080-805, Brazil.
ACS Omega. 2025 Jun 5;10(23):24520-24531. doi: 10.1021/acsomega.5c01094. eCollection 2025 Jun 17.
Due to their optimized mechanical, physicochemical, and biocompatible properties, poly-(lactic acid) (PLA)-based composites functionalized with graphene have attracted growing interest in the biomedical field. In this study, a nanocomposite was developed by incorporating graphene at different concentrations into a commercial PLA-like resin, processed using the additive manufacturing technique of vat photopolymerization. Cell viability assays using human melanoma (MV3) tumor cells were performed on samples containing 0.1 and 0.3 wt % graphene. The results showed a significant, concentration-dependent inhibition of tumor cell proliferation. In contrast, nontumor cells (MNP-01 and MRC-5) exhibited good biocompatibility with the material, reinforcing the selective behavior of the composite. To better understand the composite's anticancer potential and its interactions with cells, extensive structural, topographical, and vibrational characterizations were conducted. X-ray diffraction (XRD) analysis confirmed that the addition of graphene did not significantly alter the polymer's structural profile. Raman spectroscopy yielded similar findings, showing unchanged vibrational modes. Atomic force microscopy (AFM) revealed changes in surface roughness, while Vickers microhardness (VM) tests showed increased hardness with higher graphene content. Wettability tests supported the biological findings, indicating increased hydrophilicity with higher graphene concentrations, which correlates with decreased MV3 cell viability. These results help elucidate the mechanisms mediating the interaction between cells and the PLA/graphene composite surface.
由于其优化的机械、物理化学和生物相容性，用石墨烯功能化的聚乳酸（PLA）基复合材料在生物医学领域引起了越来越多的关注。在本研究中，通过将不同浓度的石墨烯掺入一种商业化的类PLA树脂中，采用光固化3D打印的增材制造技术制备了一种纳米复合材料。使用人黑色素瘤（MV3）肿瘤细胞对含有0.1 wt%和0.3 wt%石墨烯的样品进行了细胞活力测定。结果显示，肿瘤细胞增殖受到显著的、浓度依赖性的抑制。相比之下，非肿瘤细胞（MNP - 01和MRC - 5）与该材料表现出良好的生物相容性，强化了复合材料的选择性行为。为了更好地理解复合材料的抗癌潜力及其与细胞的相互作用，进行了广泛的结构、形貌和振动表征。X射线衍射（XRD）分析证实，石墨烯的添加并未显著改变聚合物的结构特征。拉曼光谱也得出了类似的结果，显示振动模式未发生变化。原子力显微镜（AFM）揭示了表面粗糙度的变化，而维氏显微硬度（VM）测试表明，随着石墨烯含量的增加，硬度也随之增加。润湿性测试支持了生物学研究结果，表明随着石墨烯浓度的增加，亲水性增强，这与MV3细胞活力的降低相关。这些结果有助于阐明介导细胞与PLA/石墨烯复合表面相互作用的机制。
