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1847年纳尔多所描述的肾海绵的染色体基因组序列及其相关微生物宏基因组序列。

The chromosomal genome sequence of the kidney sponge, Nardo, 1847, and its associated microbial metagenome sequences.

作者信息

Pita Lucia, Maldonado Manuel, Koutsouveli Vassiliki, Riesgo Ana, Hentschel Ute, Oatley Graeme, Sinclair Elizabeth, Aunin Eerik, Gettle Noah, Santos Camilla, Paulini Michael, Niu Haoyu, McKenna Victoria, O'Brien Rebecca

机构信息

Institute of Marine Sciences - CSIC, Barcelona, Spain.

Integrated Marine Ecology group, Institute of Marine Research IIM-CSIC, Vigo, Spain.

出版信息

Wellcome Open Res. 2025 May 29;10:283. doi: 10.12688/wellcomeopenres.24166.1. eCollection 2025.

Abstract

We present a genome assembly from a specimen of (kidney sponge; Porifera; Demospongiae; Chondrillida; Chondrillidae). The genome sequence has a total length of 117.37 megabases. Most of the assembly (99.98%) is scaffolded into 14 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 17.45 kilobases in length. Several symbiotic bacterial genomes were assembled as MAGs. Gene annotation of the host organism assembly on Ensembl identified 17,340 protein-coding genes. The metagenome of the specimen was also assembled and 53 binned bacterial genomes were identified, including 40 high-quality MAGs that were representative of a typical high microbial abundance sponge and included three candiate phyla (Poribacteria, Latescibacteria, Binatota).

摘要

我们展示了来自一种(肾海绵;多孔动物门;寻常海绵纲;软海绵目;软海绵科)标本的基因组组装。基因组序列全长117.37兆碱基。大部分组装序列(99.98%)被构建成14条染色体假分子。线粒体基因组也已组装完成,长度为17.45千碱基。几个共生细菌基因组被组装为宏基因组组装基因组(MAGs)。在Ensembl上对宿主生物组装体进行的基因注释鉴定出17340个蛋白质编码基因。该标本的宏基因组也进行了组装,鉴定出53个分箱的细菌基因组,包括40个高质量的MAGs,它们代表了典型的高微生物丰度海绵,并且包括三个候选门(多孔杆菌门、隐秘杆菌门、双纳托菌门)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc81/12181767/44ab2798a61c/wellcomeopenres-10-26657-g0000.jpg

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