Tronchin G, Schrével J
J Protozool. 1977 Feb;24(1):67-82. doi: 10.1111/j.1550-7408.1977.tb05282.x.
In an ultrastructural study the development of the sporozoite as well as the growth and development of the trophozoite of Gregarina blaberae were followed in the course of experimental infections of larvae of the cokroach Blaberus craniifer. The spectacular growth involved the transformation within 18 days of the sporozoite, measuring 15 X 1 micronm, to a cephaline--trophozoite affixed to the intestinal epithelium--of 250 micronm length and 65 micronm diameter. The sporozoite's ultrastructure is not different from that of sporozoites of other Sporozoa studies to date--the conoid and dense bodies are present. The pellicle consists of 3 membranes, but there are some interruptions in the internal membrane complex. The first dictyosomes are formed from the nuclear envelope. The migration of the nucleus and of the dense bodies, followed by the regression of all the structures characteristic of the sporozoites, and the establishment of a cortical zone that comes to cover the epimerite, take place within 48 h after infection and mark the transformation of the sporozoite into the trophozoite. Development of the cephaline involves the formation of the epicytic folds, which occurs at the base of the deutomerite, starting on the 3rd day of development. A regular system of longitudinal or epicytic folds is formed over the entire surface of the gregarine. On the 4th and 5th days of development, a vacuolar system and a chondriome become differentiated in the epimerite, while a fibrillar septum separates the protomerite from the deutomerite. The next stage, starting on the 6th day, is characterized by distribution of polysaccharide reserves between these 2 segments. The model studied allows us to determine the role of the epimerite in the parasite's nutrition, as well as the development of the chondriome and of the cortical membranes in the course of the vegetative growth phase of the cephaline gregarine.
在一项超微结构研究中，追踪了格氏隐孢球虫子孢子的发育以及滋养体在大头蜚蠊幼虫实验感染过程中的生长和发育。显著的生长过程包括在18天内，将长15×1微米的子孢子转变为附着在肠上皮的、长250微米、直径65微米的头状滋养体。该子孢子的超微结构与迄今研究的其他孢子虫的子孢子并无不同——具有类锥体和致密体。表膜由3层膜组成，但内膜复合体存在一些中断。最初的高尔基体由核膜形成。感染后48小时内，细胞核和致密体迁移，随后所有子孢子特征性结构退化，同时形成一个覆盖附器的皮质区，标志着子孢子向滋养体的转变。头状结构的发育涉及在发育第3天从后节基部开始形成表膜褶皱。在整个隐孢球虫表面形成规则的纵向或表膜褶皱系统。在发育的第4天和第5天，附器中分化出液泡系统和线粒体，同时一个纤维状隔膜将前节与后节分开。从第6天开始的下一阶段，特征是多糖储备在这两个节段之间分布。所研究的模型使我们能够确定附器在寄生虫营养中的作用，以及头状隐孢球虫营养生长阶段线粒体和皮质膜的发育情况。
