Li Weiming, Jia Yuzhen, Chen Kunyilan, Li Huifang, Yang Huaixia, Guo Liang, Miao Mingsan
Henan University of Chinese Medicine, Zhengzhou, China.
Pharmacy College, Henan University of Chinese Medicine, Zhengzhou, China.
Mikrochim Acta. 2025 Jun 23;192(7):443. doi: 10.1007/s00604-025-07217-7.
Acetamiprid (ACE), a next-generation chlorinated neonicotinoid insecticide, has been extensively employed for pest control. However, its excessive residues in food and the environment have raised significant concerns regarding human health. To address the need for a simple, accurate, and efficient ACE detection method, a novel ratiometric aptamer sensor was developed based on the dual signal amplification strategy of atom transfer radical polymerization (ATRP) and gold nanoparticles (AuNPs). The internal reference system was established by the electrostatic adsorption/embedding interaction of methylene blue (MB) with double-stranded deoxyribonucleic acid (dsDNA), and the reaction site was expanded using AuNPs loaded with multiplexed DNA sequences, which was combined with the in situ grafting of conductive polymers by ATRP to achieve signal multiplication. Under the optimized conditions, the sensor exhibited good linearity in the range 70 pg/mL ~ 300 ng/mL with a detection limit of 19.26 pg/mL. During the real sample analysis, the sensor revealed remarkable stability, reproducibility, and sensitivity in the detection of ACE residues in food. The design principle of such a sensor provides a reliable tool for accurate monitoring of pesticides.
啶虫脒（ACE）是一种新一代氯化烟碱类杀虫剂，已被广泛用于害虫防治。然而，其在食品和环境中的过量残留引发了人们对人类健康的重大担忧。为了满足对一种简单、准确且高效的ACE检测方法的需求，基于原子转移自由基聚合（ATRP）和金纳米粒子（AuNPs）的双信号放大策略，开发了一种新型的比率型适体传感器。通过亚甲基蓝（MB）与双链脱氧核糖核酸（dsDNA）的静电吸附/嵌入相互作用建立了内参体系，并使用负载有多重DNA序列的AuNPs扩展反应位点，将其与通过ATRP原位接枝导电聚合物相结合以实现信号倍增。在优化条件下，该传感器在70 pg/mL至300 ng/mL范围内表现出良好的线性，检测限为19.26 pg/mL。在实际样品分析中，该传感器在检测食品中的ACE残留时显示出显著的稳定性、重现性和灵敏度。这种传感器的设计原理为准确监测农药提供了可靠的工具。
