Pang Xiyou, Hu Yuqing, Dai Zhihui, Lou Qiuwen, Xu Wenxia, Chen Lin
Department of Anesthesiology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, Zhejiang Province, China.
Central Laboratory, Precision Medicine Center, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, Zhejiang Province, China.
Discov Oncol. 2025 Jun 23;16(1):1181. doi: 10.1007/s12672-025-02959-5.
Organoids are cultures that use stem cells to induce differentiation or clinical samples as raw materials to form three-dimensional spatial structures through specific culture systems, thereby simulating the structure and function of tissues in vivo. Organoids are characterized by their ease of construction, long-term stable passage, clinical authenticity, and application in high-throughput drug screening. Patient-derived organoids have great application value in colorectal cancer research and individualized treatment. In this review, recent advances in colorectal cancer organoid culture technology, clinical individualized drug sensitivity assessment, new drug screening, drug mechanism research, nanomedicine-based applications, and imaging technology are summarized. These advancements will provide support for the precise diagnosis and treatment of colorectal cancer and the development of new treatment strategies, which will have significant scientific value and clinical transformation value.
类器官是利用干细胞诱导分化或临床样本作为原材料，通过特定培养系统形成三维空间结构，从而模拟体内组织的结构和功能的培养物。类器官具有易于构建、长期稳定传代、临床真实性以及在高通量药物筛选中的应用等特点。患者来源的类器官在结直肠癌研究和个体化治疗中具有巨大的应用价值。在这篇综述中，总结了结直肠癌类器官培养技术、临床个体化药物敏感性评估、新药筛选、药物机制研究、基于纳米医学的应用以及成像技术等方面的最新进展。这些进展将为结直肠癌的精准诊断和治疗以及新治疗策略的开发提供支持，具有重大的科学价值和临床转化价值。
