Costa Santos Fábio Henrique de Paulo, de Melo Patrícia Santos, Borella Paulo Sérgio, Neves Flávio Domingues das, Zancope Karla
Department of Occlusion, Fixed Prosthodontics, and Dental Materials, School of Dentistry, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil.
Department of General Practice, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, United States of America.
PLoS One. 2025 Jun 23;20(6):e0325068. doi: 10.1371/journal.pone.0325068. eCollection 2025.
It is not clear the effect of multiple autoclaving processes on the accuracy of scan bodies. Autoclaving dental disposables enables reuse, reducing the environmental impact of raw materials and waste.
This in vitro laboratory study evaluated the effect of the autoclaving process on the intraoral scan body (SB) manufactured from different materials (Titanium and PEEK), positioned at the implant and abutment levels.
Two models, each with 10 implants, were created to evaluate scan body trueness made of two materials (Titanium and PEEK), and implant junction positioned at two levels (implant and abutment levels). Analyses were conducted at five distinct time points: T0 (control group with new SB), T1 (after one autoclaving cycle), T2 (10 cycles), T3 (50 cycles), and T4 (100 cycles). Forty SBs were divided into four groups (n = 10), fixed on the models according to the initial positioning, using the recommended torque, and scanned 10 times using an intraoral Sccaner (InEOS X5 scanner, Dentsply Sirona). The surface deviation was evaluated by .STL mesh overlay using an image software (Geomagic Control X software). Microscopic analyses of marginal adaptation were performed simultaneously with the digital analysis using an optical microscope (Mitutoyo™-500 Optical Microscope) with 40x magnification at four places (mesial, distal, buccal, and lingual). Statistical analysis was performed using statistical software (SPSS 29.0 software), with α = 0,05. Data were subjected to the Shapiro-Wilk normality test. To assess spatial variation after the cycles, Repeated Measures ANOVA with Bonferroni correction was used. For microscopic qualitative analysis, percentile was used to check the prevalence of clinically adapted and misfitting faces.
Statistically significant differences in mesh deformation were detected, with PEEK SB showing greater deformation compared to Titanium, especially at the abutment level, after 100 cycles (P < 0.01). Microscopic analysis revealed that 100% of the faces were classified as "Clinically Adapted" by the three calibrated evaluators (KAPPA > 0.08).
Titanium scan bodies showed less surface deviation than PEEK but both remained below 50 µm. Internal-junction scan bodies were more affected than abutment-level ones. All scan bodies stayed within acceptable limits and viable after 100 autoclaving cycles.
尚不清楚多次高压灭菌过程对扫描体精度的影响。对牙科一次性用品进行高压灭菌可实现重复使用，减少原材料和废物对环境的影响。
本体外实验室研究评估了高压灭菌过程对由不同材料（钛和聚醚醚酮）制成、位于种植体和基台水平的口腔内扫描体（SB）的影响。
创建两个模型，每个模型有10个种植体，以评估由两种材料（钛和聚醚醚酮）制成、种植体连接位于两个水平（种植体和基台水平）的扫描体的真实性。在五个不同时间点进行分析：T0（使用新SB的对照组）、T1（一次高压灭菌循环后）、T2（10个循环）、T3（50个循环）和T4（100个循环）。将40个SB分为四组（n = 10），根据初始定位固定在模型上，使用推荐扭矩，并使用口腔内扫描仪（InEOS X5扫描仪，登士柏西诺德）扫描10次。使用图像软件（Geomagic Control X软件）通过.STL网格叠加评估表面偏差。在数字分析的同时，使用放大40倍的光学显微镜（Mitutoyo™ - 500光学显微镜）在四个位置（近中、远中、颊侧和舌侧）进行边缘适应性的微观分析。使用统计软件（SPSS 29.0软件）进行统计分析，α = 0.05。数据进行Shapiro-Wilk正态性检验。为评估循环后的空间变化，使用带Bonferroni校正的重复测量方差分析。对于微观定性分析，使用百分位数检查临床适配和不匹配面的发生率。
检测到网格变形存在统计学显著差异，100个循环后，聚醚醚酮扫描体比钛扫描体显示出更大的变形，尤其是在基台水平（P < 0.01）。微观分析显示，三位校准评估者将100%的面分类为“临床适配”（KAPPA > 0.08）。
钛扫描体的表面偏差小于聚醚醚酮扫描体，但两者均保持在50 µm以下。种植体内部连接水平的扫描体比基台水平的扫描体受影响更大。所有扫描体在100次高压灭菌循环后仍保持在可接受范围内且可行。
