Sidharta Kent, Suryono Suryono, Pritia Mardha Ade, Murdiastuti Kwartarini
Clinical Dentistry Magister Program, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia.
Department of Periodontology, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia.
Eur J Dent. 2025 Jun 23. doi: 10.1055/s-0045-1809528.
Injectable platelet-rich fibrin (i-PRF) features a higher concentration of growth factor and lower viscosity compared with PRF, making it advantageous for periodontal regenerative therapy. However, its low mechanical property and high degradation rate cause its limited usage in bone augmentation. Hydrogel interacts with i-PRF, which is expected to replace bone graft considering its disadvantages. Through the synergistic effects of the materials, a sustained release of growth factor is achieved, promoting bone formation and maturation.Osteogenic markers, including alkaline phosphatase (ALP) activity and calcium deposition, were measured at intervals of 1, 7, 14, and 21 days using osteoblast-like cells. In vivo study using the extraction socket of Wistar rat applied with the same material was also done and measured at 21 and 42 days. The study included three groups: hydrogel i-PRF, bone graft (FDBA) i-PRF, and a control (blank hydrogel) group. Measurements utilized ALP staining and Alizarin red S assays for the in vitro study and bone dimension for the in vivo study.Hydrogel i-PRF significantly enhanced ALP activity on days 7 and 14 compared with the bone graft i-PRF and control groups ( ≤ 0.05). Similarly, calcium deposition was notably higher in the hydrogel i-PRF group on days 14 and 21. Hydrogel i-PRF also preserves the bone dimension of the rat's extraction socket compared with bone graft i-PRF. These findings highlight the superior bone regeneration capacity of the hydrogel when combined with i-PRF, attributed to enhanced osteoblast proliferation, differentiation, and mineralization mediated by PDGF and BMP bound to collagen fibrils.Hydrogel with i-PRF exhibits improved osteogenic capability compared with bone grafts, showing promise as an alternative material for periodontal regenerative applications.
与富血小板纤维蛋白（PRF）相比，可注射富血小板纤维蛋白（i-PRF）具有更高的生长因子浓度和更低的粘度，这使其在牙周再生治疗中具有优势。然而，其低机械性能和高降解率导致其在骨增量中的应用受限。水凝胶与i-PRF相互作用，考虑到其缺点，有望替代骨移植材料。通过材料的协同作用，实现了生长因子的持续释放，促进了骨形成和成熟。使用成骨样细胞，在第1、7、14和21天的间隔测量包括碱性磷酸酶（ALP）活性和钙沉积在内的成骨标志物。还使用相同材料对Wistar大鼠的拔牙窝进行了体内研究，并在第21天和42天进行了测量。该研究包括三组：水凝胶i-PRF组、骨移植（FDBA）i-PRF组和对照组（空白水凝胶）。体外研究采用ALP染色和茜素红S测定法，体内研究采用骨尺寸测量法。与骨移植i-PRF组和对照组相比，水凝胶i-PRF在第7天和第14天显著增强了ALP活性（P≤0.05）。同样，在第14天和第21天，水凝胶i-PRF组的钙沉积明显更高。与骨移植i-PRF相比，水凝胶i-PRF还保留了大鼠拔牙窝的骨尺寸。这些发现突出了水凝胶与i-PRF结合时卓越的骨再生能力，这归因于与胶原纤维结合的血小板衍生生长因子（PDGF）和骨形态发生蛋白（BMP）介导的成骨细胞增殖、分化和矿化增强。与骨移植相比，含i-PRF的水凝胶表现出更好的成骨能力，有望作为牙周再生应用的替代材料。
