Zizmare Laimdota, Hofmann Ute, Jarboui Mohamed Ali, Klose Franziska, Fraschka Sabine, Matthes Jakob, Krüger Marcel, Schaeffeler Elke, Schwab Matthias, Ueffing Marius, Pichler Bernd J, Boldt Karsten, Casadei Nicolas, Trautwein Christoph
Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tübingen, 72076, Tübingen, Germany.
Cluster of Excellence iFIT (EXC2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tübingen, 72076, Tübingen, Germany.
Sci Rep. 2025 Jun 23;15(1):20254. doi: 10.1038/s41598-025-06438-3.
The classical approach of using adjacent pieces of fresh-frozen tissue for various omics analysis from the same sample possesses a risk of biological mismatch between arising from intrinsic tissue heterogeneity. We propose an alternative approach of tissue cryogenic pulverization and lyophilization before distribution for omics studies for a more reliable analysis. Here, we compare individual omics layer readouts from fresh-frozen adjacent tissue pieces and homogenized powder in mouse brain, kidney, and liver. Genomics, transcriptomics, proteomics, and metabolomics analyses showed comparable RNA integrity, DNA methylation, and coverage of transcripts, proteins, and metabolites across both methods. Moreover, the homogenized-lyophilized powder usage led to reduced heterogeneity between biological replicates. We conclude that the cryogenically pulverized-lyophilized tissue approach not only maintains a critical molecular feature coverage and quality but also provides a homogenous basis for various omics analysis enhancing reproducibility, sample transport, storage and enabling multi omics base on one and the same tissue aliquot.
使用同一样本的相邻新鲜冷冻组织进行各种组学分析的经典方法，存在因内在组织异质性而产生生物学不匹配的风险。我们提出了一种替代方法，即在进行组学研究之前对组织进行低温粉碎和冻干，以进行更可靠的分析。在此，我们比较了小鼠脑、肾和肝中新鲜冷冻的相邻组织块和匀浆粉末的各个组学层面读数。基因组学、转录组学、蛋白质组学和代谢组学分析表明，两种方法在RNA完整性、DNA甲基化以及转录本、蛋白质和代谢物的覆盖范围方面具有可比性。此外，使用匀浆冻干粉末可降低生物学重复之间的异质性。我们得出结论，低温粉碎冻干组织方法不仅能保持关键分子特征的覆盖范围和质量，还为各种组学分析提供了一个均匀的基础，提高了可重复性、样本运输和存储能力，并能够基于同一组织等分试样进行多组学分析。
