demonstration and characterization of a condensed, reverse TCA (crTCA) cycle.

INTRODUCTION

Plants employ the Calvin-Benson cycle (CBC) to fix atmospheric CO for the production of biomass. The flux of carbon through the CBC is limited by the activity and selectivity of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO). Alternative CO fixation pathways that do not use RuBisCO to fix CO have evolved in some anaerobic, autotrophic microorganisms.

METHODS

Rather than modifying existing routes of carbon metabolism in plants, we have developed a synthetic carbon fixation cycle that does not exist in nature but is inspired by metabolisms of bacterial autotrophs. In this work, we build and characterize a condensed, reverse tricarboxylic acid (crTCA) cycle and .

RESULTS

We demonstrate that a simple, synthetic cycle can be used to fix carbon in vitro under aerobic and mesophilic conditions and that these enzymes retain activity whenexpressed transiently . We then evaluate stable transgenic lines of that have both phenotypic and physiologic changes. Transgenic are shorter than controls with increased rates of photosynthetic CO assimilation and changes in photorespiratory metabolism.

DISCUSSION

This first iteration of a build-test-learn phase of the crTCA cycle provides promising evidence that this pathway can be used to increase photosynthetic capacity in plants.