Xu De-Tian, Chen Qi, Yang Jia-Yi, Yan Guo-Rong, Zhang Ling-Lin, Liu Xiao-Jing, Wang Pei-Ru, Liu Jia, Wang Xiu-Li
Shanghai Skin Disease Hospital, Tongji University Medical School, Shanghai, China.
The Ice Dermalab, Shanghai, China.
Front Immunol. 2025 Jun 9;16:1566786. doi: 10.3389/fimmu.2025.1566786. eCollection 2025.
Skin microecology significantly affects health, with the microbiome being a complex community of microorganisms. Different niche preferences of microorganisms raise concerns about the adequacy of common sampling methods like swabbing and cyanoacrylate biopsy. In this study, we aim to contribute to a more suitable sampling strategy in acne microbiome studies.
This study involved ten mild to moderate acne patients. Three sampling methods were used: swab sampling (S1), modified standardized skin surface biopsy (S2), and individual comedo extraction (S3). DNA was extracted and sequenced to analyze the microbiome data.
There were significant differences in the bacterial and fungal microbiome data obtained by the three different sampling methods. spp. (significantly higher in S3, <0.05) and spp. (higher in S3, <0.05) were most affected by sampling methods. Bacterial phyla Proteobacteria (abundant in S1) and Bacteroidota (dominant in S2) also showed method-dependent variations.
The choice of sampling method significantly impacts microbiome data, highlighting the need for accurate sampling to understand the relationship between the skin microbiome and acne. Standardizing sampling methods in future studies is essential for advancing skin microecology research.
http://www.chictr.org.cn, identifier ChiCTR-CPC-17012398.
皮肤微生物群对健康有重大影响，微生物组是一个复杂的微生物群落。微生物不同的生态位偏好引发了对诸如拭子采样和氰基丙烯酸酯活检等常用采样方法是否充分的担忧。在本研究中，我们旨在为痤疮微生物组研究提供一种更合适的采样策略。
本研究纳入了10名轻至中度痤疮患者。使用了三种采样方法：拭子采样（S1）、改良标准化皮肤表面活检（S2）和单个粉刺提取（S3）。提取DNA并进行测序以分析微生物组数据。
三种不同采样方法获得的细菌和真菌微生物组数据存在显著差异。 spp.（在S3中显著更高，<0.05）和 spp.（在S3中更高，<0.05）受采样方法影响最大。细菌门变形菌门（在S1中丰富）和拟杆菌门（在S2中占主导）也表现出方法依赖性变化。
采样方法的选择对微生物组数据有显著影响，这凸显了准确采样对于理解皮肤微生物组与痤疮之间关系的必要性。在未来研究中标准化采样方法对于推进皮肤微生物生态学研究至关重要。
