Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.
Syst Appl Microbiol. 2023 May;46(3):126405. doi: 10.1016/j.syapm.2023.126405. Epub 2023 Mar 5.
Strain LMG 31809 was isolated from a top soil sample of a temperate, mixed deciduous forest in Belgium. Comparison of its 16S rRNA gene sequence with that of type strains of bacteria with validly published names positioned it in the class Alphaproteobacteria and highlighted a major evolutionary divergence from its near neighbor species which represented species of the orders Emcibacterales and Sphingomonadales. 16S rRNA amplicon sequencing of the same soil sample revealed a highly diverse community in which Acidobacteria and Alphaproteobacteria predominated, but failed to yield amplicon sequence variants highly similar to that of strain LMG 31809 . There were no metagenome assembled genomes that corresponded to the same species and a comprehensive analysis of public 16S rRNA amplicon sequencing data sets demonstrated that strain LMG 31809 represents a rare biosphere bacterium that occurs at very low abundances in multiple soil and water-related ecosystems. The genome analysis suggested that this strain is a strictly aerobic heterotroph that is asaccharolytic and uses organic acids and possibly aromatic compounds as growth substrates. We propose to classify LMG 31809 as a novel species within a novel genus, Govania unica gen. nov., sp. nov, within the novel family Govaniaceae of the class Alphaproteobacteria. Its type strain is LMG 31809 (=CECT 30155 ). The whole-genome sequence of strain LMG 31809 has a size of 3.21 Mbp. The G + C content is 58.99 mol%. The 16S rRNA gene and whole-genome sequences of strain LMG 31809 are publicly available under accession numbers OQ161091 and JANWOI000000000, respectively.
LMG 31809 菌株从比利时温带落叶混交林的表土样本中分离得到。将其 16S rRNA 基因序列与具有有效发表名称的细菌的模式菌株进行比较，将其定位在α变形菌纲，与近邻种（代表肠杆菌目和鞘氨醇单胞菌目）存在显著的进化分歧。对同一土壤样本的 16S rRNA 扩增子测序显示，该群落中优势菌为酸杆菌和α变形菌，但未能产生与 LMG 31809 菌株高度相似的扩增子序列变体。没有宏基因组组装基因组与同一物种相对应，对公共 16S rRNA 扩增子测序数据集的全面分析表明，LMG 31809 菌株代表了一种罕见的生物圈细菌，在多个土壤和水相关生态系统中丰度极低。基因组分析表明，该菌株是一种严格需氧异养菌，具有非发酵性，以有机酸和可能的芳香化合物为生长底物。我们建议将 LMG 31809 菌株分类为一个新属 Govania 属的新种，即 Govania unica 种，nov，属于 α变形菌纲的 Govaniaceae 科。其模式菌株为 LMG 31809 菌株（=CECT 30155 菌株）。LMG 31809 菌株的全基因组序列大小为 3.21 Mbp，G+C 含量为 58.99 mol%。LMG 31809 菌株的 16S rRNA 基因和全基因组序列分别可在 OQ161091 和 JANWOI000000000 号登录号下获取。
