Huang Ruixu, Kratka Caroline E, Pea Jeffrey, McCann Cai, Nelson Jack, Bryan John P, Zhou Luhan T, Russo Daniela D, Zaniker-Gomez Emily J, Gandhi Achla H, Shalek Alex K, Cleary Brian, Farhi Samouil L, Duncan Francesca E, Goods Brittany A
Thayer School of Engineering at Dartmouth College, Hanover, New Hampshire, United States of America.
Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
PLoS Biol. 2025 Jun 24;23(6):e3003193. doi: 10.1371/journal.pbio.3003193. eCollection 2025 Jun.
Ovulation is a spatiotemporally coordinated process that involves several tightly controlled events, including oocyte meiotic maturation, cumulus expansion, follicle wall rupture and repair, and ovarian stroma remodeling. To date, no studies have detailed the precise window of ovulation at single-cell resolution. Here, we performed parallel single-cell RNA-seq and spatial transcriptomics on paired mouse ovaries across an ovulation time course to map the spatiotemporal profile of ovarian cell types. We show that major ovarian cell types exhibit time-dependent transcriptional states enriched for distinct functions and have specific localization profiles within the ovary. We also identified gene markers for ovulation-dependent cell states and validated these using orthogonal methods. Finally, we performed cell-cell interaction analyses to identify ligand-receptor pairs that may drive ovulation, revealing previously unappreciated interactions. Taken together, our data provides a rich and comprehensive resource of murine ovulation that can be mined for discovery by the scientific community.
排卵是一个时空协调的过程，涉及多个严格控制的事件，包括卵母细胞减数分裂成熟、卵丘扩张、卵泡壁破裂与修复以及卵巢基质重塑。迄今为止，尚无研究以单细胞分辨率详细描述排卵的精确时间窗。在此，我们在排卵时间进程中对配对的小鼠卵巢进行了平行单细胞RNA测序和空间转录组学分析，以绘制卵巢细胞类型的时空图谱。我们发现，主要的卵巢细胞类型呈现出随时间变化的转录状态，这些状态富含不同的功能，并且在卵巢内具有特定的定位模式。我们还鉴定了依赖于排卵的细胞状态的基因标志物，并使用正交方法对其进行了验证。最后，我们进行了细胞-细胞相互作用分析，以确定可能驱动排卵的配体-受体对，揭示了以前未被认识到的相互作用。综上所述，我们的数据提供了丰富而全面的小鼠排卵资源，可供科学界挖掘发现。
