Thangprasert Atsadaporn, Bernhardt Anne, Gossla Elke, Kilian David, Klaiklay Saranyoo, Meesane Jirut, Srithep Papitchaya, Gelinsky Michael, Pasitsuparoad Pakorn
Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Muang Surat Thani 84000, Thailand.
Centre for Translation Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Faculty of Medicine of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
Biomed Mater. 2025 Jul 3;20(4). doi: 10.1088/1748-605X/ade7e4.
The treatment of osteochondral defects is a major topic of current research and has become more important in our aging society. The challenges in bone and cartilage repair arise from the structure and function of these different tissues. This study proposes a biphasic model combining cartilage and bone scaffolds based on silk fibroin (SF) biopolymers. For the cartilage phase, SF scaffolds were coated with gelatin and/or agarose layers. For bone scaffolds, mineralized collagen solutions were coated on or mixed into the SF matrix. The physical and biological properties of these samples were evaluated to find the optimum conditions for a biphasic scaffold. Modification of both cartilage and bone scaffolds resulted in a smaller pore size, lower swelling rate, and higher rigidity. Gelatin significantly promoted cartilage biomarker production and agarose facilitated cell proliferation, inducing a homogeneous cell distribution and stimulating chondrogenesis. Furthermore, modification with mineralized collagen decreased cell proliferation in osteoblast progenitors but enhanced differentiation into osteoblasts. The optimum conditions were found to be a mixture of gelatin and agarose for the coating in the cartilage phase and low mineralized collagen content for the bone phase.
骨软骨缺损的治疗是当前研究的一个主要课题，在我们这个老龄化社会中变得越发重要。骨和软骨修复面临的挑战源于这些不同组织的结构和功能。本研究提出了一种基于丝素蛋白（SF）生物聚合物的软骨和骨支架相结合的双相模型。对于软骨阶段，SF支架涂覆有明胶和/或琼脂糖层。对于骨支架，将矿化胶原溶液涂覆在SF基质上或混入其中。对这些样品的物理和生物学特性进行评估，以找到双相支架的最佳条件。软骨和骨支架的改性均导致孔径减小、溶胀率降低和刚性提高。明胶显著促进软骨生物标志物的产生，琼脂糖促进细胞增殖，诱导细胞均匀分布并刺激软骨形成。此外，用矿化胶原进行改性会降低成骨祖细胞的增殖，但会增强其向成骨细胞的分化。发现最佳条件是软骨阶段涂层采用明胶和琼脂糖的混合物，骨阶段采用低矿化胶原含量。
