Elkin R G, Griffith J E
J Assoc Off Anal Chem. 1985 Sep-Oct;68(5):1028-32.
Corn, peanut meal, and soybean meal samples were either untreated or oxidized with performic acid before hydrolysis; the amino acids were determined by cation exchange high performance liquid chromatography (LC) and conventional cation exchange LC using an amino acid analyzer (AAA). Reproducibility of each procedure was assessed by repeated injections of the same calibration standard solution over a period of several days. LC data were more precise with regard to coefficients of variation for amino acid retention times, but were more variable with regard to peak areas. Although some significant differences between methods were noted, feedstuff amino acid values obtained by LC and AAA compared very well. The only consistent differences observed within each feedstuff were that Phe and Tyr values were significantly lower when analyzed by LC compared with AAA. Results of this study suggest that modular LC instrumentation can be used to accurately and reproducibly analyze amino acids in feedstuff hydrolysates. Advantages of using ninhydrin derivatization for feedstuff analysis, as opposed to using o-phthalaldehyde or dansyl chloride, are discussed.
玉米、花生粕和大豆粕样品在水解前要么未经处理，要么用过甲酸氧化；氨基酸通过阳离子交换高效液相色谱（LC）和使用氨基酸分析仪（AAA）的传统阳离子交换LC进行测定。通过在几天时间内重复进样相同的校准标准溶液来评估每个程序的重现性。就氨基酸保留时间的变异系数而言，LC数据更精确，但就峰面积而言，其变异性更大。尽管注意到方法之间存在一些显著差异，但通过LC和AAA获得的饲料氨基酸值比较吻合。在每种饲料中观察到的唯一一致差异是，与AAA相比，通过LC分析时苯丙氨酸和酪氨酸的值显著较低。本研究结果表明，模块化LC仪器可用于准确且可重复地分析饲料水解产物中的氨基酸。讨论了与使用邻苯二甲醛或丹磺酰氯相比，使用茚三酮衍生化进行饲料分析的优点。
