Elkin R G
J Assoc Off Anal Chem. 1984 Sep-Oct;67(5):1024-6.
Corn, soybean meal, and isolated soybean protein samples were acid-hydrolyzed and analyzed for amino acid content by reverse phase liquid chromatography (LC) and by conventional ion-exchange chromatography (IEC) using an amino acid analyzer. The former method employed pre-column derivatization with orthophthalaldehyde (OPTA)/ethanethiol and fluorescence detection. In the LC procedure, glycine and threonine were not resolved, and proline and cyst(e)ine were not detected. In general, amino acid values obtained by LC and IEC compared closely within and across feedstuffs, and both agreed well with published amino acid composition data. The notable exceptions were aspartic acid, glutamic acid, and alanine. Results of this study suggest that reverse phase LC with pre-column OPTA derivatization can be applied to accurately measure primary amino acids in individual feedstuffs.
将玉米、豆粕和分离大豆蛋白样品进行酸水解，然后使用氨基酸分析仪通过反相液相色谱法（LC）和传统离子交换色谱法（IEC）分析氨基酸含量。前一种方法采用邻苯二甲醛（OPTA）/乙硫醇进行柱前衍生化和荧光检测。在LC分析过程中，甘氨酸和苏氨酸未能分离，脯氨酸和半胱氨酸未被检测到。总体而言，通过LC和IEC获得的氨基酸值在不同饲料原料之间比较接近，并且两者都与已发表的氨基酸组成数据吻合良好。值得注意的例外是天冬氨酸、谷氨酸和丙氨酸。本研究结果表明，采用柱前OPTA衍生化的反相LC可用于准确测定单一饲料原料中的初级氨基酸。
