Özentürk U, Genç M, Ömür A D, Erbaş E, Uysal A, Yörü A, Genç A, Akarsu S A
Department of Animal Science, Faculty of Veterinary Medicine, Atatürk University, 25010 Erzurum, Turkey.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Atatürk University, 25010 Erzurum, Turkey.
Pol J Vet Sci. 2025 Jun;28(2):175-181. doi: 10.24425/pjvs.2025.154026.
In this study, the effects of in-ovo injection of Chrysin (CR) into Japanese quail eggs on testicular histology, oxidant status and epididymal sperm quality were investigated. 720 Japanese quail eggs were divided into 4 groups and 0.1 mL saline was given to the control group, 0.25 mg CR to the 0.25 group, 0.50 mg CR to the 0.50 group and 0.75 mg CR to the 0.75 group. On the 60th day after the laying of eggs, 8 Japanese quails were randomly selected from each group and sacrificed under mild sevoflurane anesthesia. The abdominal cavity was opened and both testicular tissues and epididymal parts were removed. The right testicular tissue was used for histopathological examinations and the left one was used for biochemical analyses. The epididymal part at the tip of the vas deferens at the end of the right testis was trimmed in 100 μL saline at 37°C. Histopathological examinations showed that histological scoring was higher in the CR 50 and CR 75 groups. A decrease in oxidant status was observed in all CR groups compared to the control group. Spermatozoa density was higher in CR groups compared to the control group. Total motility value was statistically significantly higher in CR 50 and CR 75 groups compared to control and CR 25 groups. There was no statistical difference between the groups in terms of dead and abnormal spermatozoa. In-ovo CR injection at doses of 0.50 and 0.75 mg/egg improved testicular histological score, decreased oxidative stress, and increased epididymal sperm quality.
在本研究中，研究了对日本鹌鹑蛋进行卵内注射白杨素（CR）对睾丸组织学、氧化状态和附睾精子质量的影响。720枚日本鹌鹑蛋被分为4组，对照组注射0.1 mL生理盐水，0.25组注射0.25 mg CR，0.50组注射0.50 mg CR，0.75组注射0.75 mg CR。在产蛋后的第60天，从每组中随机选取8只日本鹌鹑，在轻度七氟醚麻醉下处死。打开腹腔，取出双侧睾丸组织和附睾部分。右侧睾丸组织用于组织病理学检查，左侧用于生化分析。将右侧睾丸末端输精管尖端的附睾部分在37℃的100 μL生理盐水中修剪。组织病理学检查显示，CR 50组和CR 75组的组织学评分更高。与对照组相比，所有CR组的氧化状态均降低。与对照组相比，CR组的精子密度更高。与对照组和CR 25组相比，CR 50组和CR 75组的总活力值在统计学上显著更高。各组之间的死精和畸形精子方面无统计学差异。以0.50和0.75 mg/蛋的剂量进行卵内CR注射可改善睾丸组织学评分，降低氧化应激，并提高附睾精子质量。
