Ruiz Roberta Tognareli, Batista Aline Cechinel Assing, Maria Ruiz Jorge Luis
Laboratório de Biotecnologia Aplica à Saúde. Universidade Federal da Integração Latino-Americana - UNILA, Foz do Iguaçú, PR, Brazil.
Programa de Pós-graduação em Patologia Experimental e Comparada. Universidade de São Paulo. SP, Brazil.
Braz J Vet Med. 2025 Jun 13;47:e001425. doi: 10.29374/2527-2179.bjvm001425. eCollection 2025.
is a parasite that causes leishmaniasis in its visceral clinical manifestations, which is considered a zoonosis and can infect both humans and animals. Currently, there is no highly effective treatment available, and many animals that exhibit symptoms ultimately die as a result of the disease and its complications. The clinical signs of leishmaniasis are varied and nonspecific. The main symptoms are severe anemia and thrombocytopenia, weight loss, splenomegaly, lymphadenomegaly, liver disease, kidney failure, and skin lesions, among others. Due to the chronic inflammatory state caused by the parasite, an oxidative environment is created, leading to potential cell injury and damage to the infected animals' genetic material. To investigate DNA damage, we conducted the micronucleus test and comet assay, as well as measured serum LDH levels in infected and non-infected dogs. Our results indicate that infected dogs present significantly higher levels of serum LDH (461.4 ± 204.5 U/L, n=36) compared to healthy dogs (142.38 ± 37.94 U/L, n=5). Additionally, the DNA of infected dogs is more damaged than that of the control group, as demonstrated by the micronucleus test (p=0.01) and comet assay (p=0.002). These findings suggest that infection can lead to clastogenic events, highlighting the need for further research on this process. It is important to consider the potential mutagenic properties of , given its ability to cause DNA damage in infected animals.
是一种寄生虫，可导致内脏利什曼病的临床表现，利什曼病被认为是一种人畜共患病，可感染人类和动物。目前，尚无高效治疗方法，许多出现症状的动物最终因该病及其并发症而死亡。利什曼病的临床症状多样且无特异性。主要症状包括严重贫血、血小板减少、体重减轻、脾肿大、淋巴结肿大、肝病、肾衰竭和皮肤病变等。由于寄生虫引起的慢性炎症状态，会形成氧化环境，导致潜在的细胞损伤以及感染动物遗传物质的损害。为了研究DNA损伤，我们进行了微核试验和彗星试验，并测量了感染和未感染犬的血清乳酸脱氢酶（LDH）水平。我们的结果表明，与健康犬（142.38±37.94 U/L，n=5）相比，感染犬的血清LDH水平显著更高（461.4±204.5 U/L，n=36）。此外，微核试验（p=0.01）和彗星试验（p=0.002）表明感染犬的DNA损伤比对照组更严重。这些发现表明感染可导致染色体断裂事件，凸显了对此过程进行进一步研究的必要性。鉴于其在感染动物中导致DNA损伤的能力，考虑其潜在的诱变特性很重要。
