Juntree Nichapat, Sawatdee Sopanat, Pongchaikul Pisut, Arjfuk Pariyapat, Wanmolee Wanwitoo, Khemthong Pongtanawat, Chanlek Narong, Srifa Atthapon, Posoknistakul Pattaraporn, Laosiripojana Navadol, Wu Kevin C W, Sakdaronnarong Chularat
Department of Chemical Engineering, Faculty of Engineering, Mahidol University, 999 Putthamonthon 4 Road, Salaya, Putthamonthon, Nakhon Pathom 73170, Thailand.
Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, 111 Suwannabhumi Canal Road, Bang Pla, Bang Phli District, Samut Prakan 10540, Thailand.
ACS Meas Sci Au. 2025 Apr 7;5(3):304-324. doi: 10.1021/acsmeasuresciau.5c00011. eCollection 2025 Jun 18.
Diabetes affects over 8.8% of the global population, driving demand for noninvasive glucose detection methods. Traditional enzymatic assays are sensitive but face challenges such as high cost, complex preparation, low stability, and enzyme denaturation. This study aimed to enhance glucose detection sensitivity with a noninvasive easy-to-use technique using a fluorescent cellulose film. Lignin-derived carbon dots (LCDs) were synthesized as cost-effective, stable nanozymes for fluorescence-based glucose sensing. It was found that doping noble metal Ru onto Pt/LCDs synthesized in water mimicked peroxidase enzyme and could enhance the reactivity and sensitivity to ultralow levels for glucose detection at room temperature. To fabricate a wearable sensor, a transparent cellulose film embedded with PtRu/LCDs and glucose oxidase (GOx) was fabricated for biocompatible glucose sensing. The film achieved sensitive detection in the range of 0.05-1.0 mM ( = 0.94) with a detection limit of 50 μM, suitable for noninvasive glucose detection in saliva, tears, and sweat. This study highlights the potential of the PtRu/LCD-based cellulose film for highly sensitive, wearable glucose sensors compatible with smartphone applications, offering a simple, real-time, noninvasive, fast, and chemical reagent-free glucose sensing for preventive healthcare.
糖尿病影响着全球超过8.8%的人口，推动了对非侵入性血糖检测方法的需求。传统的酶法检测灵敏度高，但面临着成本高、制备复杂、稳定性低和酶变性等挑战。本研究旨在通过使用荧光纤维素膜的非侵入性易用技术提高血糖检测灵敏度。合成了木质素衍生的碳点（LCDs）作为具有成本效益、稳定的纳米酶用于基于荧光的葡萄糖传感。研究发现，在水中合成的Pt/LCDs上掺杂贵金属Ru可模拟过氧化物酶，并能在室温下提高对超低水平葡萄糖检测的反应性和灵敏度。为了制造可穿戴传感器，制备了一种嵌入PtRu/LCDs和葡萄糖氧化酶（GOx）的透明纤维素膜用于生物相容性葡萄糖传感。该膜在0.05 - 1.0 mM范围内实现了灵敏检测（ = 0.94），检测限为50 μM，适用于唾液、眼泪和汗液中的非侵入性葡萄糖检测。本研究突出了基于PtRu/LCDs的纤维素膜在与智能手机应用兼容的高灵敏度、可穿戴葡萄糖传感器方面的潜力，为预防性医疗保健提供了一种简单、实时、非侵入性、快速且无需化学试剂的葡萄糖传感方法。
