Zhang Ruilin, Liu Lei, Li Wei, Luo Xiaogang, Wu Fengshou
Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430072, PR China.
Hubei Key Laboratory of Biomass Fibers and Eco-Dyeing & Finishing, Wuhan Textile University, Wuhan 430200, PR China.
Colloids Surf B Biointerfaces. 2023 Feb;222:113125. doi: 10.1016/j.colsurfb.2023.113125. Epub 2023 Jan 3.
The luminescent carbon dots with peroxidase mimicking property had attracted considerable attention in biomedical field. In this work, iron-doped carbon dots (Fe-CDs) were prepared by one-pot hydrothermal method with 5, 10, 15, 20-tetra (4-borate phenyl)-21H, 23H-porphyrin Fe (II) (Fe-TBPP) as precursor. The obtained Fe-CDs emitted intense blue luminescence under ultraviolet light irradiation. Moreover, the Fe-CDs exhibited remarkable peroxidase mimicking property, which can efficiently catalyze the oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB) into blue ox-TMB in the presence of hydrogen peroxide (HO). More importantly, the emission of Fe-CDs could be gradually quenched with the addition of HO. Based on these phenomena, a new optical dual-mode (colorimetric and fluorometric) method for the detection of HO and glucose was successfully established. The detection limits of glucose were calculated to be 3.86 and 7.27 μM (S/N = 3) respectively based on the colorimetric and fluorometric methods. Furthermore, we combined this dual-mode detection method with smartphone imaging. The colorimetric and fluorescent images were collected by recognition software of smartphone, which were then transformed into the corresponding HSL values for quantitative determination of glucose. Finally, the dual-mode approach based on Fe-CDs was used for the detection of glucose content in human serum, demonstrating the potential application of carbon dots in the biological area.
具有过氧化物酶模拟特性的发光碳点在生物医学领域引起了广泛关注。在本工作中，以5,10,15,20-四（4-硼酸苯基）-21H,23H-卟啉铁（II）（Fe-TBPP）为前驱体，通过一锅水热法制备了铁掺杂碳点（Fe-CDs）。所制备的Fe-CDs在紫外光照射下发出强烈的蓝色荧光。此外，Fe-CDs表现出显著的过氧化物酶模拟特性，在过氧化氢（H₂O₂）存在下，能有效地催化3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色的氧化态TMB（ox-TMB）。更重要的是，随着H₂O₂的加入，Fe-CDs的荧光会逐渐猝灭。基于这些现象，成功建立了一种用于检测H₂O₂和葡萄糖的新型光学双模式（比色法和荧光法）方法。基于比色法和荧光法计算得到葡萄糖的检测限分别为3.86和7.27 μM（S/N = 3）。此外，我们将这种双模式检测方法与智能手机成像相结合。通过智能手机的识别软件收集比色和荧光图像，然后将其转换为相应的HSL值用于葡萄糖的定量测定。最后，基于Fe-CDs的双模式方法用于检测人血清中的葡萄糖含量，证明了碳点在生物领域的潜在应用。
