Adisti Meisa Zalfa, Widjiati Widjiati, Sunarso Agus, Luqman Epy Muhammad, Suprayogi Tri Wahyu, Madyawati Sri Pantja, Utama Suzanita, Firmawati Aulia
Student of Postgraduate Biology Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo, Indonesia.
Departement of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo, Surabaya, Indonesia.
Open Vet J. 2025 May;15(5):2004-2008. doi: 10.5455/OVJ.2025.v15.i5.15. Epub 2025 May 31.
Vitrification of oocytes can lead to the formation of reactive oxygen species during the maturation and warming processes. In this study, there is a comparison between the use of commercial cryoprotectant and ethylene glycol + sucrose to observe the antioxidant activity that arises during oxidative stress, namely superoxide dismutase (SOD). SOD is produced to counteract free radicals that are damaging to cells; the higher the level of SOD in a cell, the further the cell is from damage. Improving oocyte cryopreservation techniques contributes to greater reproductive efficiency in livestock, which plays an important role in supporting sustainable and effective food production.
The aim of this study is to determine whether different cryoprotectant use can affect the intracellular SOD levels in oocytes.
This study is an experimental laboratory study using goat oocytes divided into 3 treatment groups: control (K) goat oocytes without vitrification, Treatment 1 (P1) goat oocytes vitrified using a commercial cryoprotectant, and Treatment 2 (P2) goat oocytes vitrified using ethylene glycol and 1-M sucrose. All groups were vitrified for 1 week, followed by a warming process. Then, the SOD levels were calculated using SOD ELISA kit.
The results showed that this study has a significance level ( < 0.05) with the control group K having the lowest SOD levels and P2 having the highest SOD levels.
Therefore, it can be concluded that the use of ethylene glycol as a cryoprotectant can maintain SOD levels in oocyte cells.
卵母细胞玻璃化冷冻在成熟和复温过程中可导致活性氧的形成。在本研究中，对使用商业冷冻保护剂与乙二醇+蔗糖进行了比较，以观察氧化应激期间产生的抗氧化活性，即超氧化物歧化酶（SOD）。SOD的产生是为了对抗对细胞有损害的自由基；细胞中SOD水平越高，细胞离损伤越远。改进卵母细胞冷冻保存技术有助于提高家畜的繁殖效率，这对支持可持续和有效的粮食生产起着重要作用。
本研究的目的是确定不同冷冻保护剂的使用是否会影响卵母细胞内的SOD水平。
本研究是一项实验性实验室研究，使用山羊卵母细胞，分为3个处理组：对照组（K）为未进行玻璃化冷冻的山羊卵母细胞，处理1组（P1）为使用商业冷冻保护剂进行玻璃化冷冻的山羊卵母细胞，处理2组（P2）为使用乙二醇和1-M蔗糖进行玻璃化冷冻的山羊卵母细胞。所有组均玻璃化冷冻1周，随后进行复温过程。然后，使用SOD ELISA试剂盒计算SOD水平。
结果表明，本研究具有显著性水平（<0.05），对照组K的SOD水平最低，P2组的SOD水平最高。
因此，可以得出结论，使用乙二醇作为冷冻保护剂可以维持卵母细胞中的SOD水平。
