Timcenko-Youssef L, Yamazaki R K, Kimura T
J Biol Chem. 1985 Oct 25;260(24):13355-9.
In view of the physiological importance of adrenocortical lipid peroxidation, we have carried out subcellular fractionation to determine the location of glutathione peroxidase, an enzyme which protects against lipid peroxidation. Glutathione peroxidase is present in both cytosolic (92%) and mitochondrial (8%) fractions. The small activity in mitochondria is not due to contamination by the cytosolic activity as evidenced by several rigorous approaches. The mitochondrial enzyme is located in the matrix and appears to be effective in protection from NADPH-dependent lipid peroxidative damage of cytochrome P-450 and succinic dehydrogenase, which are located exclusively in the inner membrane.
鉴于肾上腺皮质脂质过氧化的生理重要性，我们进行了亚细胞分级分离，以确定谷胱甘肽过氧化物酶的位置，该酶可防止脂质过氧化。谷胱甘肽过氧化物酶存在于胞质溶胶（92%）和线粒体（8%）组分中。线粒体中的少量活性并非由胞质溶胶活性污染所致，这已通过多种严格方法得到证实。线粒体酶位于基质中，似乎能有效保护位于内膜的细胞色素P-450和琥珀酸脱氢酶免受NADPH依赖性脂质过氧化损伤。
