Klimek J, Schaap A P, Kimura T
Biochem Biophys Res Commun. 1983 Jan 27;110(2):559-66. doi: 10.1016/0006-291x(83)91186-5.
The relationship between NADPH-dependent lipid peroxidation and the degradation of cytochrome P-450 has been studied in bovine adrenal cortex mitochondria. Malondialdehyde formation is accompanied by a corresponding decrease in total cytochrome P-450 content. Inhibitors of lipid peroxidation also prevent the loss of cytochrome P-450, further demonstrating a direct relationship between NADPH-dependent lipid peroxidation and degradation of P-450. To differentiate between cytochrome P-450(11)beta and P-450scc, steroid-induced difference spectra were used to evaluate P-450 degradation. These measurements provide the first evidence that both P-450's are degraded during NADPH-dependent lipid peroxidation with P-450(11)beta being much more susceptible to this process.
已在牛肾上腺皮质线粒体中研究了NADPH依赖性脂质过氧化与细胞色素P - 450降解之间的关系。丙二醛的形成伴随着细胞色素P - 450总含量相应降低。脂质过氧化抑制剂也可防止细胞色素P - 450的损失，进一步证明了NADPH依赖性脂质过氧化与P - 450降解之间存在直接关系。为了区分细胞色素P - 450(11)β和P - 450scc，使用类固醇诱导差异光谱来评估P - 450的降解。这些测量提供了首个证据，即两种P - 450在NADPH依赖性脂质过氧化过程中均会降解，其中P - 450(11)β对该过程更为敏感。
