Xiao Kunqin, Li Yalan, Gu Songyang, Liu Ling, Zhang Guiping, Zhang Yanhua, Zhang Xianghui, Liu Jinliang, Pan Hongyu
College of Plant Sciences, Jilin University, Changchun 130062, China.
College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
J Agric Food Chem. 2025 Jul 9;73(27):16787-16803. doi: 10.1021/acs.jafc.5c04925. Epub 2025 Jun 25.
The understanding of drug resistance mechanisms beyond increasing drug efflux and modifying drug targets remains limited. Here, we revealed that the GATA-type transcription repressor SsGATA2 negatively regulated the drug resistance of , a devastating phytopathogenic fungus, to various fungicides. In mechanism, the deletion of led to the derepression of the expression of several enzymes responsible for synthesizing cell wall and membrane components, inducing the constitutive thickening of the cell wall and improved cell membrane stability, which eventually prevented fungicides from entering and conferred notable fungicide resistance. Furthermore, SsGATA2 plays a positive role in regulating mycelial growth, sclerotium development, infection cushion formation, nutrient assimilation, and pathogenicity in . Our work demonstrates that transcription repressor SsGATA2 negatively regulates broad-spectrum resistance to fungicides and positively contributes to morphogenesis and pathogenicity. These findings reveal a strategy through which phytopathogenic fungi resist fungicides by fortifying the cell wall and membrane.
除了增加药物外排和修饰药物靶点之外，对耐药机制的理解仍然有限。在这里，我们揭示了GATA型转录抑制因子SsGATA2负向调节一种毁灭性植物病原真菌对各种杀菌剂的耐药性。在机制上，SsGATA2的缺失导致负责合成细胞壁和膜成分的几种酶的表达去抑制，诱导细胞壁的组成型增厚并提高细胞膜稳定性，最终阻止杀菌剂进入并赋予显著的抗杀菌剂能力。此外，SsGATA2在调节菌丝生长、菌核发育、侵染垫形成、养分同化和致病性方面发挥积极作用。我们的工作表明，转录抑制因子SsGATA2负向调节对杀菌剂的广谱抗性，并对形态发生和致病性有积极贡献。这些发现揭示了一种植物病原真菌通过强化细胞壁和细胞膜来抵抗杀菌剂的策略。
