College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Pestic Biochem Physiol. 2018 Jul;149:61-66. doi: 10.1016/j.pestbp.2018.05.010. Epub 2018 May 26.
Pyrisoxazole is a pyridine compound of demethylation inhibitor fungicides. In this study, baseline sensitivity of Sclerotinia sclerotiorum to pyrisoxazole was determined using 166 strains from the oilseed rape fields in 2014, 2015 and 2016. The EC values for mycelial growth inhibition ranged from 0.0214 to 0.5443 μg mL, with a mean EC value of 0.2329 ± 0.1048 μg mL and were normally distributed. The EC values had no significant difference among three populations from 2014, 2015, and 2016. There was no correlation with sensitivity between pyrisoxazole and carbendazim or iprodione. After treated with pyrisoxazole, we observed increased cell membrane permeability, and decreased exopolysaccharide and oxalic acid production, which can contribute to reduced virulence of S. sclerotiorum and lead to failure of disease infection. Protective and curative activity tests showed that pyrisoxazole exhibited excellent protective and curative activity against S. sclerotiorum in oilseed rape, and protective activity was better than curative activity. Compared with the currently used fungicides, pyrisoxazole not only exhibited excellent control efficacy on Sclerotinia stem rot, but also dramatically reduced the doses of fungicides in the field trials. Overall, these data provide more references for revealing pharmacological effect of pyrisoxazole against S. sclerotiorum and managing Sclerotinia stem rot on oilseed rape caused by benzimidazole- and dicarboximide-resistant populations.
吡唑肟是一种脱甲基抑制剂类吡啶化合物。本研究于 2014、2015 和 2016 年，采用油菜田采集的 166 个菌系，测定了核盘菌对吡唑肟的基础敏感性。菌丝生长抑制的 EC 值范围为 0.0214-0.5443μg/mL，平均值为 0.2329±0.1048μg/mL，呈正态分布。2014、2015 和 2016 年 3 个菌系的 EC 值之间无显著差异。吡唑肟与多菌灵或异菌脲之间的敏感性无相关性。用吡唑肟处理后，我们观察到细胞膜通透性增加，胞外多糖和草酸产量减少，这有助于降低核盘菌的毒力，导致病害侵染失败。保护和治疗活性测试表明，吡唑肟对油菜核盘菌表现出优异的保护和治疗活性，保护活性优于治疗活性。与目前使用的杀菌剂相比，吡唑肟不仅对菌核病具有优异的防治效果，而且在田间试验中显著减少了杀菌剂的用量。总体而言，这些数据为揭示吡唑肟对核盘菌的药理作用以及管理由苯并咪唑类和二羧酰亚胺类抗性种群引起的油菜菌核病提供了更多参考。
