希腊产KPC-2、SHV-106、CTX-M-15和VEB-1的耐碳青霉烯类高风险ST307克隆株报告。
Report of High-Risk Carbapenem-Resistant ST307 Clone Producing KPC-2, SHV-106, CTX-M-15, and VEB-1 in Greece.
作者信息
Chatzidimitriou Maria, Tsolakidou Pandora, Kyriazidi Maria Anna, Varlamis Sotiris, Frydas Ilias S, Mavridou Maria, Mitka Stella
机构信息
Department of Biomedical Sciences, International Hellenic University, 57400 Thessaloniki, Greece.
Microbiology Department, Hospital of Volos, Polymeri 134, 38222 Volos, Greece.
出版信息
Antibiotics (Basel). 2025 May 31;14(6):567. doi: 10.3390/antibiotics14060567.
BACKGROUND/OBJECTIVES: ST307 is emerging as a significant global high-risk antimicrobial-resistant (AMR) clone with a notable capacity to acquire and disseminate resistance genes. However, there is limited research on the pathogenicity, virulence, and adaptation of ST307 strains and on the clinical characteristics of infected patients.
METHODS
In this study, a carbapenem-resistant ST307 strain named U989 was isolated from a urine culture of a hospitalized patient in Volos, Greece, in July 2024. Whole-genome sequencing was performed to identify resistance genes to β-lactams , , , , , and and resistance genes to other antibiotics.
RESULTS
A genomic analysis also revealed the presence of virulence factors such as A, K1, A, H, A, 2, and T and an IncFiB(pQil)/IncFII(K) replicon, which harbors the gene. Additionally, the transposable element Tn4401 was identified as a key vehicle for the mobilization of the resistance gene. Finally, this is the report of a high-risk ST307 clone expressing KPC-2, SHV-106, CTX-M-15, and VEB-1 genes in Greece.
CONCLUSIONS
The coexistence of these resistance genes in addition to aminoglycoside, quinolone, and other resistance genes results in difficult-to-treat infections caused by respective carrier strains, often requiring the use of last-resort antibiotics and contributing to the global challenge of antimicrobial resistance.
背景/目的：ST307正成为一种重要的全球高风险抗菌药物耐药（AMR）克隆，具有获取和传播耐药基因的显著能力。然而，关于ST307菌株的致病性、毒力和适应性以及感染患者的临床特征的研究有限。
方法
在本研究中，2024年7月从希腊沃洛斯一名住院患者的尿液培养物中分离出一株名为U989的耐碳青霉烯类ST307菌株。进行全基因组测序以鉴定对β-内酰胺类、、、、、和的耐药基因以及对其他抗生素的耐药基因。
结果
基因组分析还揭示了毒力因子如A、K1、A、H、A、2和T的存在以及一个IncFiB(pQil)/IncFII(K)复制子，其携带基因。此外，转座子Tn4401被确定为耐药基因转移的关键载体。最后，这是希腊一份关于表达KPC-2、SHV-106、CTX-M-15和VEB-1基因的高风险ST307克隆的报告。
结论
这些耐药基因与氨基糖苷类、喹诺酮类和其他耐药基因共存，导致各自携带菌株引起难以治疗的感染，常常需要使用最后手段的抗生素，并加剧了全球抗菌药物耐药性挑战。
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