Noh Jin Hee, Ahn Ji Yong, Na Hee Kyong, Lee Jeong Hoon, Jung Kee Wook, Kim Do Hoon, Choi Kee Don, Song Ho June, Lee Gin Hyug, Jung Hwoon-Yong
Department of Internal Medicine, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang 14068, Republic of Korea.
Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea.
Antibiotics (Basel). 2025 Jun 5;14(6):580. doi: 10.3390/antibiotics14060580.
: (HP) and Epstein-Barr virus (EBV) coinfection lead to chronic inflammation and contribute to the development of gastric cancer. However, studies examining the association between HP virulence factors and EBV infection in gastric cancer are limited. This study investigated the polymorphisms of HP virulence factors associated with EBV infection and their effects on clinical outcomes in EBV-associated gastric cancer (EBVaGC). : A total of 96 HP isolates from 54 patients with gastric cancer were divided and analyzed based on EBV coinfection status. Polymerase chain reaction amplifications of virulence factors were conducted using DNA extracts from HP isolates cultured from gastric mucosal specimens. : EBV infection was significantly associated with gastric carcinoma with lymphoid stroma morphology and a proximal location in the stomach. Most HP strains from patients with gastric cancer were positive for (100.0%), (100.0%), and 1 (87.5%). Among HP isolates with EBV coinfection, the prevalence of 2 (21.7% vs. 0.0%, < 0.001) and (21.7% vs. 4.0%, = 0.009) was significantly more frequent, and that of 1 (78.3% vs. 96.0%, = 0.009) and s1a (4.3% vs. 22.0%, = 0.012) was less frequent than those of EBV- colonies. Multivariate analysis indicated that (odds ratio, 6.148; 95% confidence interval [CI], 1.221 to 30.958; = 0.028) was associated with EBVaGC. No significant difference in clinical outcomes was observed based on the presence of expression in EBVaGC. : In gastric cancer, regardless of EBV infection, most HP strains were highly virulent, testing positive for , , and 1. Although was significantly associated with EBV infection, it did not influence the clinical outcomes of EBVaGC.
幽门螺杆菌（HP）与爱泼斯坦-巴尔病毒（EBV）共同感染会导致慢性炎症，并促进胃癌的发生。然而，研究幽门螺杆菌毒力因子与胃癌中EBV感染之间关联的研究有限。本研究调查了与EBV感染相关的幽门螺杆菌毒力因子多态性及其对EBV相关胃癌（EBVaGC）临床结局的影响。：从54例胃癌患者中分离出的96株幽门螺杆菌，根据EBV共同感染状态进行分组和分析。使用从胃黏膜标本培养的幽门螺杆菌分离株的DNA提取物进行毒力因子的聚合酶链反应扩增。：EBV感染与具有淋巴间质形态且位于胃近端的胃癌显著相关。胃癌患者的大多数幽门螺杆菌菌株 （100.0%）、 （100.0%）和1 （87.5%）检测呈阳性。在与EBV共同感染的幽门螺杆菌分离株中，2 （21.7%对0.0%，<0.001）和 （21.7%对4.0%，=0.009）的流行率显著更高，而1 （78.3%对96.0%，=0.009）和s1a （4.3%对22.0%，=0.012）的流行率低于EBV阴性菌落。多因素分析表明， （比值比，6.148；95%置信区间[CI]，1.221至30.958；=0.028）与EBVaGC相关。基于EBVaGC中 表达的存在，未观察到临床结局的显著差异。：在胃癌中，无论EBV感染情况如何，大多数幽门螺杆菌菌株都具有高毒力， 、 和1检测呈阳性。尽管 与EBV感染显著相关，但它并未影响EBVaGC的临床结局。
