Heyman P W, Cho C S, McRea J C, Olsen D B, Kim S W
J Biomed Mater Res. 1985 Apr;19(4):419-36. doi: 10.1002/jbm.820190407.
Heparin immobilization chemistry using alkyl spacer arms was adapted to optimize yield on polyurethane (PU) surfaces. The resultant biological activity of immobilized heparin (HI) was examined in vitro and in vivo, and compared with a heparin releasing (HR) system. Immobilized heparin retained its ability to bind and inactivate thrombin and Factor Xa; nonspecific coagulation factor binding was insignificant. Such activity cannot be attributed to the leakage of improperly bound heparin. Immobilized heparin-polyurethane catheters implanted in canine femoral and jugular veins for 1 h periods exhibited significant reduction in thrombus formation compared with untreated PU contralateral controls. Polyurethane catheters coated with a 9% heparin dispersion in PU (HR) system provided even greater improvement in antithrombogenicity.
采用烷基间隔臂的肝素固定化学方法，以优化聚氨酯（PU）表面的产量。对固定化肝素（HI）的体外和体内生物活性进行了检测，并与肝素释放（HR）系统进行了比较。固定化肝素保留了其结合和灭活凝血酶及因子Xa的能力；非特异性凝血因子结合不显著。这种活性不能归因于结合不当的肝素的泄漏。与未处理的对侧PU对照相比，植入犬股静脉和颈静脉1小时的固定化肝素 - 聚氨酯导管血栓形成显著减少。涂有9%肝素在PU中的分散体（HR）系统的聚氨酯导管在抗血栓形成方面有更大的改善。
