Dewanjee M K, Rowland S M, Kapadvanjwala M, Hellman R L, MacGregor D C, Serafini A N, Palatianos G M, Georgiou M F, Eckstein E C, Sfakianakis G N
University of Miami, School of Medicine, Department of Radiology, Florida 33101.
ASAIO Trans. 1990 Jul-Sep;36(3):M745-9.
The dynamics of platelet deposition and embolization from control and heparin bonded polyurethane catheters (CPC and HBPC) was evaluated with In-111 labeled autologous platelets (IN-PLT) and a computerized gamma camera (CGC). Ten non-heparinized dogs (18-25 kg) were catheterized in both femoral arteries with 10 cm of CPC and HBPC (5 Fr., Cordis) 24 hr after injection of 300-420 microCi of In-PLT, and imaged for 3 hr with the computerized gamma camera. The regional platelet deposition curves (RPDC) indicated multiple peaks and valleys; the curves were analyzed for early rate of thrombus formation (upswing), thrombus retention time (full width at half maxima of the RPDC-peak), and rate of embolization (downswing) on both catheters. The four parameters (mean +/- SD) of thrombosis on catheters and integral of the radioactivity time curve for the 3 hr duration of imaging were calculated from normalized counts/sec. The rate of thrombus formation and rate of embolization are higher for the control than HBPC, suggesting that heparin-bonding decreases the early rate of thrombosis and embolization. The thrombus adhesivity and retention time appear shorter for the control catheter, indicating that the control thrombogenic catheter forms multiple thrombi and emboli than HBPC. The integral appears larger for the control catheter than HBPC. In vivo (dynamic) studies, in vitro studies, and critical analyses of the radioactivity time curve were essential for complete evaluation of thrombogenicity of catheters and other cardiovascular prostheses.
使用铟 - 111标记的自体血小板（IN - PLT）和计算机化γ相机（CGC）评估了对照和肝素结合聚氨酯导管（CPC和HBPC）的血小板沉积和栓塞动力学。在注入300 - 420微居里的IN - PLT后24小时，将10只未肝素化的犬（18 - 25千克）的双侧股动脉分别插入10厘米长的CPC和HBPC（5F，Cordis），并用计算机化γ相机成像3小时。局部血小板沉积曲线（RPDC）显示出多个峰谷；分析了两条导管上血栓形成的早期速率（上升段）、血栓保留时间（RPDC峰的半高宽）和栓塞速率（下降段）。根据归一化计数/秒计算导管上血栓形成的四个参数（平均值±标准差）以及成像3小时期间放射性时间曲线的积分。对照导管的血栓形成速率和栓塞速率高于HBPC，这表明肝素结合降低了血栓形成和栓塞的早期速率。对照导管的血栓粘附性和保留时间似乎更短，这表明对照血栓形成导管比HBPC形成更多的血栓和栓子。对照导管的积分似乎比HBPC更大。体内（动态）研究、体外研究以及对放射性时间曲线的关键分析对于全面评估导管和其他心血管假体的血栓形成性至关重要。
