Yan Ruhui, Zhao Yuewu, Geng Huaixiao, Yan Mengxia, Wang Jine, Han Shuang
School of Science, Shenyang University of Chemical Technology, Shenyang 110042, China.
Shandong Provincial Engineering Laboratory of Novel Pharmaceutical Excipients, College of Pharmacy, Sustained and Controlled Release Preparations, Dezhou University, Dezhou 253023, China.
Biosensors (Basel). 2025 May 30;15(6):348. doi: 10.3390/bios15060348.
Micron-sized, ultrathin metal-organic framework (MOF) sheet is a two-dimensional (2D) hybrid material with a large specific surface area, which can be used not only in the fields of energy and biomedicine, but also in electrode modification to improve the electrochemical detection effect. In this work, the 2D-structured Co-TCPP(Fe) MOF sheets were synthesized from porphyrin molecules and cobalt ions and then combined with reduced graphene oxide (rGO) and perfluorosulfonic acid polymer (Nafion) solution to construct Co-TCPP(Fe)/rGO/Nafion-modified electrodes capable of sensitively capturing dopamine (DA). The 2D ultrathin lamellar structure of this electrode-modified material is beneficial to the formation of π-π stacking effect with DA molecules, and the oxygen-containing groups carried on its surface can also form electrostatic attraction with the amino groups of DA molecules. Therefore, the Co-TCPP(Fe)/rGO/Nafion-modified electrode under the synergistic effect shows a specific adsorption effect on DA molecules, resulting in high anti-interference ability and a low detection limit of 0.014 µM in the concentration range of 0.1-100 µM. Furthermore, the Co-TCPP(Fe)/rGO/Nafion composite material composed of micron-sized, ultrathin lamellar structures also shows high reusability due to the stability of its coordination structure and can demonstrate good results when applied to the actual sample detection of human urine.
微米级超薄金属有机框架（MOF）片是一种具有大比表面积的二维（2D）杂化材料，不仅可用于能源和生物医学领域，还可用于电极修饰以提高电化学检测效果。在本工作中，由卟啉分子和钴离子合成了二维结构的Co-TCPP(Fe) MOF片，然后与还原氧化石墨烯（rGO）和全氟磺酸聚合物（Nafion）溶液结合，构建了能够灵敏捕获多巴胺（DA）的Co-TCPP(Fe)/rGO/Nafion修饰电极。这种电极修饰材料的二维超薄层状结构有利于与DA分子形成π-π堆积效应，其表面携带的含氧基团也能与DA分子的氨基形成静电吸引。因此，在协同作用下，Co-TCPP(Fe)/rGO/Nafion修饰电极对DA分子表现出特异性吸附作用，导致高抗干扰能力，在0.1 - 100 µM浓度范围内检测限低至0.014 µM。此外，由微米级超薄层状结构组成的Co-TCPP(Fe)/rGO/Nafion复合材料由于其配位结构的稳定性还表现出高可重复使用性，并且在应用于人尿实际样品检测时能显示出良好结果。
