Suslov Maksim
Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia.
Cells. 2025 Jun 14;14(12):902. doi: 10.3390/cells14120902.
Investigating the contribution and interaction of water transport pathways in plant roots is important for understanding the functioning of the root hydraulic system. In this study, the real-time dynamics of lateral water transport along the cell-to-cell pathway and the diffusional water permeability of cells in the root suction zone of whole maize plants were investigated non-invasively by spin-echo NMR in response to rapid blockage of root apoplast. Apoplast blockage was carried out by insoluble precipitates using an original approach based on alternate incubation of whole plant roots in aqueous solutions of K[Fe(CN)] and CuSO. In the first stage after the apoplast blockage, the water transport along the cell-to-cell pathway and the diffusional water permeability of root cells was decreased 2.5 times. Using inhibitory analysis and gene expression analysis, it was shown that root aquaporins are involved in the decrease in cell-to-cell water transport in response to apoplast blockage. After an initial decrease, the cell-to-cell water transport was restored to initial values. At the same time, there was a partial compensation of the transpiration loss caused by the apoplast blockage. It is assumed that the apoplastic water flow in plant roots can modulate the cell-to-cell water transport and functional activity of aquaporins.
研究植物根系中水分运输途径的贡献和相互作用对于理解根系水力系统的功能非常重要。在本研究中，通过自旋回波核磁共振技术，以非侵入性方式研究了全株玉米根系吸水区沿细胞间途径的横向水分运输实时动态以及细胞的扩散水渗透性，以响应根部质外体的快速堵塞。质外体堵塞是通过使用一种基于将全株根系交替培养在K[Fe(CN)]和CuSO水溶液中的原始方法，利用不溶性沉淀物来实现的。在质外体堵塞后的第一阶段，沿细胞间途径的水分运输和根细胞的扩散水渗透性降低了2.5倍。通过抑制分析和基因表达分析表明，根水通道蛋白参与了质外体堵塞后细胞间水分运输的减少。在最初的减少之后，细胞间水分运输恢复到初始值。同时，质外体堵塞引起的蒸腾损失得到了部分补偿。据推测，植物根系中的质外体水流可以调节细胞间水分运输和水通道蛋白的功能活性。
