Wlaź Piotr, Socała Katarzyna, Palusińska-Szysz Marta, Świderska Urszula, Szczypior Dominika, Krasowska Magdalena, Szuster-Ciesielska Agnieszka
Department of Animal Physiology and Pharmacology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, 20-032 Lublin, Poland.
Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, 20-032 Lublin, Poland.
Cells. 2025 Jun 17;14(12):914. doi: 10.3390/cells14120914.
Allergic conditions have surged to unprecedented levels globally, affecting approximately 30% of the population. Fungi are among the major sources of allergens, accounting for about 6% of respiratory issues. Identifying the causes of respiratory allergies is not always possible. Our study assessed the capacity of two plant parasites, and , which infect and , to provoke inflammatory and asthmatic reactions in mouse models of acute and chronic asthma. We performed experiments by sensitizing mice through intranasal challenges with extracts from and . Subsequently, we used ELISA tests to measure pro-inflammatory cytokines, including IL-4, IL-5, IL-13, TNF-α, and TGF-β. We evaluated specific IgE production through ELISA and examined histological changes in mouse lungs using hematoxylin-eosin staining. Our research revealed that and induced significant production of all tested cytokines, increased specific IgE levels, and caused histological changes characteristic of acute and chronic asthma progression. Although weaker than the reference allergen ovalbumin, and possess proinflammatory and asthma-inducing capabilities, indicating the potential to expand the current list of fungal allergens.
全球过敏性疾病已飙升至前所未有的水平，影响着约30%的人口。真菌是主要的过敏原来源之一，约占呼吸道疾病的6%。确定呼吸道过敏的原因并非总是可行的。我们的研究评估了两种植物寄生虫（分别感染[具体植物1]和[具体植物2]）在急性和慢性哮喘小鼠模型中引发炎症和哮喘反应的能力。我们通过用[植物1]和[植物2]的提取物对小鼠进行鼻内激发致敏来开展实验。随后，我们使用酶联免疫吸附测定（ELISA）测试来测量促炎细胞因子，包括白细胞介素-4（IL-4）、白细胞介素-5（IL-5）、白细胞介素-13（IL-13）、肿瘤坏死因子-α（TNF-α）和转化生长因子-β（TGF-β）。我们通过ELISA评估特异性免疫球蛋白E（IgE）的产生，并使用苏木精-伊红染色检查小鼠肺部的组织学变化。我们的研究表明，[植物1]和[植物2]诱导了所有测试细胞因子的显著产生，提高了特异性IgE水平，并引起了急性和慢性哮喘进展特征性的组织学变化。尽管[植物1]和[植物2]比参考过敏原卵清蛋白的作用弱，但它们具有促炎和诱发哮喘的能力，这表明有可能扩大目前的真菌过敏原清单。
