Zhang Yuanzhe, Yao Tianyu, Xu Ru, Ma Pei, Zhao Jing, Mi Yu
Engineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China.
Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China.
Gels. 2025 Jun 10;11(6):445. doi: 10.3390/gels11060445.
Traumatic non-compressible hemorrhage and subsequent wound management remain critical challenges in military and civilian settings to this day. Cryogels have emerged as promising hemostatic materials for non-compressible hemorrhage due to their blood-triggered shape recovery. In this study, a biodegradable and mechanically resilient cryogel (CF/PD) was produced via cryopolymerization, employing methacrylated recombinant collagen as a macromolecular crosslinker alongside poly (ethylene glycol) diacrylate (PEGDA) and dopamine methacrylate (DMA). With its interpenetrating macro-porous structure and high hydrophilicity, the CF/PD rapidly absorbs blood and returns to its original shape within 1.5 s. In a rat liver defect model, CF/PD outperformed commercially available gelatin sponges, reducing hemostasis time by 74.4% and blood loss by 76.5%. Moreover, CF/PD cryogels facilitate in situ tissue regeneration by virtue of the bioactivity and degradability of recombinant collagen. This work establishes a bioactive recombinant collagen-driven cryogel platform, offering a transformative solution for managing non-compressible hemorrhage while enabling tissue regeneration.
创伤性不可压缩出血及后续伤口处理至今仍是军事和民用领域的关键挑战。由于其血液触发的形状恢复特性，冷冻凝胶已成为用于不可压缩出血的有前景的止血材料。在本研究中，通过冷冻聚合制备了一种可生物降解且具有机械弹性的冷冻凝胶（CF/PD），使用甲基丙烯酸化重组胶原蛋白作为大分子交联剂，同时使用聚（乙二醇）二丙烯酸酯（PEGDA）和甲基丙烯酸多巴胺（DMA）。CF/PD具有互穿大孔结构和高亲水性，能迅速吸收血液并在1.5秒内恢复其原始形状。在大鼠肝缺损模型中，CF/PD优于市售明胶海绵，止血时间减少74.4%，失血量减少76.5%。此外，CF/PD冷冻凝胶凭借重组胶原蛋白的生物活性和可降解性促进原位组织再生。这项工作建立了一个生物活性重组胶原蛋白驱动的冷冻凝胶平台，为处理不可压缩出血并实现组织再生提供了变革性解决方案。
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