Vojvodić Mira, Pešić Brankica, Mitrović Petar, Marjanović Jeromela Ana, Vico Ivana, Bulajić Aleksandra
Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia.
Institute of Pesticides and Environmental Protection, Banatska 31b, 11080 Belgrade, Serbia.
J Fungi (Basel). 2025 May 26;11(6):410. doi: 10.3390/jof11060410.
From 2013 to 2021, 112 binucleate spp. (BNR) isolates were obtained from the strawberry, tomato, pepper, bean, apple, cherry, meadow grass, and soil previously cropped with strawberries from 16 locations in Serbia. Morphological and molecular analyses (ITS, LSU rDNA, , tef-1α, and ) confirmed infections caused by four BNR AGs: AG-G on the cherry (globally new host), bean, and tomato; AG-U on meadow grass (globally new host) and apple, AG-A on the strawberry (the most frequently isolated), and AG-F on pepper. ITS sequence analysis revealed 24 haplotypes within the worldwide population of BNR AG-A, with Serbian isolates belonging to nine. The aggressiveness of AG-A (ten isolates), AG-G (three isolates), AG-F (one isolate), and AG-U (two isolates) was tested on seedlings of 14 hosts from Poaceae, Brassicaceae, Solanaceae, Asteraceae, Fabaceae, Cucurbitaceae, Apiaceae, and Chenopodiaceae, and on detached leaf petioles of the strawberry, tomato, sunflower, and bean, as well as on two pea cultivars. Sunflower and sugar beet were the most susceptible, with AG-G being the most aggressive and AG-A the least aggressive. AG-A could not infect cabbage, while at least one isolate of each remaining AG infected all tested hosts. The consistency between seedling and petiole tests highlights the latter as a rapid method for evaluating the pathogenicity and aggressiveness of BNR isolates.
2013年至2021年期间，从塞尔维亚16个地点的草莓、番茄、辣椒、豆类、苹果、樱桃、草地草以及之前种植过草莓的土壤中获得了112株双核菌属（BNR）分离株。形态学和分子分析（ITS、LSU rDNA、tef-1α等）证实了由四种BNR融合群引起的感染：樱桃（全球新寄主）、豆类和番茄上的AG-G；草地草（全球新寄主）和苹果上的AG-U；草莓上最常分离到的AG-A；辣椒上的AG-F。ITS序列分析揭示了BNR AG-A全球种群中的24个单倍型，塞尔维亚分离株属于其中9个。对来自禾本科、十字花科、茄科、菊科、豆科、葫芦科、伞形科和藜科的14种寄主的幼苗，以及草莓、番茄、向日葵和豆类的离体叶柄，还有两个豌豆品种，测试了AG-A（10个分离株）、AG-G（3个分离株）、AG-F（1个分离株）和AG-U（2个分离株）的致病力。向日葵和甜菜最易感，其中AG-G致病力最强，AG-A致病力最弱。AG-A不能感染卷心菜，而其余每个融合群至少有一个分离株能感染所有测试寄主。幼苗试验和叶柄试验结果的一致性突出了叶柄试验是评估BNR分离株致病性和致病力的快速方法。
