Li Zhi, Yu Junjie, Yu Mina, Cao Huijuan, Song Tianqiao, Wang Shuchen, Qi Zhongqiang, Du Yan, Pan Xiayan, Liu Yongfeng
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
J Fungi (Basel). 2025 May 31;11(6):426. doi: 10.3390/jof11060426.
Rice false smut (RFS), caused by (teleomorph: ), is a devastating fungal disease that severely impacts global rice production by reducing both yield and grain quality. While the mating-type gene is known to regulate both sexual and asexual reproduction in its regulatory mechanism remains unclear. In this study, an interacting protein of UvMAT1-1-3, a putative O-methyltransferase (UvPomt), was identified using yeast two-hybrid screening, and its interaction was further confirmed by co-localization microscopy. A quantitative reverse transcription PCR (qRT-PCR) analysis showed a significant up-regulation of expression during the early infection stage of . Functional characterization revealed that Δ mutants exhibited reduced fungal pathogenicity, vegetative growth, conidial production, and stress adaptation. Furthermore, a Western blot analysis revealed that the UvMAT1-1-3 protein level was reduced in Δ mutants, whereas the UvPomt protein level was elevated in Δ mutants. Taken together, these findings suggest a potential reciprocal regulation between UvPomt and UvMAT1-1-3. Understanding 's function could provide a potential molecular target for controlling RFS disease.
稻曲病（RFS）由[病原菌学名（有性型：[有性型学名]）]引起，是一种毁灭性的真菌病害，通过降低产量和谷粒品质严重影响全球水稻生产。虽然已知交配型基因[基因名称]在[病原菌名称]中调控有性和无性繁殖，但其调控机制仍不清楚。在本研究中，通过酵母双杂交筛选鉴定出假定的O-甲基转移酶UvPomt作为UvMAT1-1-3的相互作用蛋白，并通过共定位显微镜进一步证实了它们的相互作用。定量逆转录PCR（qRT-PCR）分析表明，在[病原菌名称]早期感染阶段[基因名称]表达显著上调。功能表征显示，Δ[突变体名称]突变体表现出真菌致病性、营养生长、分生孢子产生和胁迫适应性降低。此外，蛋白质免疫印迹分析表明，Δ[突变体名称]突变体中UvMAT1-1-3蛋白水平降低，而Δ[突变体名称]突变体中UvPomt蛋白水平升高。综上所述，这些发现表明UvPomt和UvMAT1-1-3之间可能存在相互调控。了解[基因名称]的功能可为控制稻曲病提供潜在的分子靶点。
