Xue Mengyao, Hou Xuwen, Gu Gan, Dong Jie, Yang Yonglin, Pan Xiaoqian, Zhang Xuan, Xu Dan, Lai Daowan, Zhou Ligang
Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
J Fungi (Basel). 2023 Dec 31;10(1):31. doi: 10.3390/jof10010031.
(anamorph: ) is the pathogen of rice false smut (RFS), which is a destructive rice fungal disease. The albino strain LN02 is a natural white-phenotype mutant of due to its incapability to produce toxic ustilaginoidins. In this study, three strains including the normal strain P1, albino strain LN02, and complemented strain -1 of the LN02 strain were employed to investigate the activation of the ustilaginoidin biosynthesis gene in the albino strain LN02 to influence sporulation, conidia germination, pigment production, stress responses, and the inhibition of rice seed germination. The activation of the ustilaginoidin biosynthesis gene increased fungal tolerances to NaCl-induced osmotic stress, Congo-red-induced cell wall stress, SDS-induced cell membrane stress, and HO-induced oxidative stress. The activation of also increased sporulation, conidia germination, pigment production, and the inhibition of rice seed germination. In addition, the activation of was able to increase the mycelial growth of the albino strain LN02 at 23 °C and a pH from 5.5 to 7.5. The findings help in understanding the effects of the activation of in albino strain LN02 on development, pigment production, stress responses, and the inhibition of rice seed germination by controlling ustilaginoidin biosynthesis.
（无性型：）是稻曲病的病原菌，稻曲病是一种具有破坏性的水稻真菌病害。白化菌株LN02是由于其无法产生有毒的类黑粉菌素而形成的自然白色表型突变体。在本研究中，使用了包括正常菌株P1、白化菌株LN02以及LN02菌株的互补菌株-1在内的三种菌株，来研究白化菌株LN02中类黑粉菌素生物合成基因的激活对孢子形成、分生孢子萌发、色素产生、胁迫反应以及水稻种子萌发抑制的影响。类黑粉菌素生物合成基因的激活提高了真菌对NaCl诱导的渗透胁迫、刚果红诱导的细胞壁胁迫、SDS诱导的细胞膜胁迫以及H₂O₂诱导的氧化胁迫的耐受性。该基因的激活还增加了孢子形成、分生孢子萌发、色素产生以及对水稻种子萌发的抑制作用。此外，该基因的激活能够在23℃和pH值为5.5至7.5的条件下增加白化菌株LN02的菌丝体生长。这些发现有助于理解在白化菌株LN02中激活该基因通过控制类黑粉菌素生物合成对发育、色素产生、胁迫反应以及水稻种子萌发抑制的影响。
