-Based Bioformulation to Control Wilt of Caused by .

In recent years, there has been extensive documentation of pathogenic fungi infecting . However, investigations of microorganisms with antagonistic properties against these pathogens are limited. This study successfully isolated two isolates of the genus (F16 and F19) from the rhizosphere soil of fruiting bodies, both of which exhibit potent antagonistic activity against , the causative agent of wilt disease. Comprehensive characterization, including physiological-biochemical tests and 16S rDNA sequence analysis, led to the identification of these isolates as sp. F16 and sp. F19. Both isolates significantly inhibited through multiple mechanisms. The volatile compounds produced by these isolates effectively suppressed the conidial germination of in vitro. Furthermore, fermentation filtrates at various dilutions exhibited pronounced antifungal activity against conidial germination, with sp. F16 showing 66.93% inhibition at a 50× dilution and sp. F19 achieving 49.22% inhibition under identical conditions. Field experiments have demonstrated the practical applicability of these antagonists. The topical application of fermentation filtrates (diluted 50×) from both isolates significantly reduced the incidence and severity of disease in cultivation. Notably, the yield improvements were substantial: fields treated with sp. F16 produced 299.6 g/m, whereas those treated with sp. F19 yielded 277.65 g/m. These yields significantly surpassed those of both the untreated control group (231 g/m) and the -inoculated group (134.93 g/m). These findings indicate that the two isolates not only effectively control but also increase the yield of .