Reis Ana, Sarmento Maria João, Ferreira Mariana, Gameiro Paula, de Freitas Victor
Rede de Quimica e Tecnologia-Laboratorio Associado de Quimica Verde, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal.
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal.
Membranes (Basel). 2025 May 24;15(6):159. doi: 10.3390/membranes15060159.
As a surface-located molecule in biological membranes, cholesterol sulphate (CholS) plays a major role in membrane-driven cell-cell processes and events including platelet-cell adhesion, T-cell receptor signalling, sperm-egg interaction, membrane fusion, and skin differentiation. Despite this, little is known about the biophysical implications of CholS at the membrane in cells and organelles. In this study, we investigate the effect of increasing the content of CholS on the biophysical properties in cholesterol-poor and cholesterol-rich biomimetic models. Data obtained show that increasing amounts of CholS result in a slight increase in anisotropy, evidence for decreased membrane fluidity at higher CholS content (10 mol%) in cholesterol-poor systems but only negligible in rigidified epithelial-like cholesterol-rich systems. On the other hand, incorporation of CholS has an overall increasing ordering effect on membrane organisation and on-surface potential that is influenced by the lipid composition and cholesterol content. Though further research is needed to gain better insights on the (patho)physiological levels of CholS in cells and organelles, our findings are discussed in the context of diet-microbiota-host interactions in membrane-driven events in inflammatory-related disorders.
作为生物膜中一种位于表面的分子，硫酸胆固醇（CholS）在膜驱动的细胞间过程和事件中发挥着重要作用，这些过程和事件包括血小板-细胞黏附、T细胞受体信号传导、精子-卵子相互作用、膜融合以及皮肤分化。尽管如此，关于CholS在细胞和细胞器膜中的生物物理影响，我们所知甚少。在本研究中，我们研究了增加CholS含量对胆固醇含量低和胆固醇含量高的仿生模型生物物理性质的影响。获得的数据表明，CholS含量增加会导致各向异性略有增加，这证明在胆固醇含量低的体系中，较高的CholS含量（10摩尔%）会使膜流动性降低，但在刚性化的富含胆固醇的上皮样体系中，这种影响微不足道。另一方面，CholS的掺入对膜组织和表面电位具有总体增强的有序化作用，这种作用受脂质组成和胆固醇含量的影响。尽管需要进一步研究以更好地了解CholS在细胞和细胞器中的（病理）生理水平，但我们在炎症相关疾病中膜驱动事件的饮食-微生物群-宿主相互作用的背景下讨论了我们的发现。
