Bouari-Coblișan Alina Petronela, Pop Claudia Felicia, Sas Valentina, Borcău Adina Georgiana, Bonci Teodora Irina, Cherecheș-Panța Paraschiva
General Nursing Discipline, Faculty of Nursing and Health Sciences, Iuliu Hațieganu University of Medicine and Pharmacy, 400124 Cluj-Napoca, Romania.
3rd Pediatric Clinic, Children's Emergency Clinical Hospital, 400315 Cluj-Napoca, Romania.
Nurs Rep. 2025 May 30;15(6):196. doi: 10.3390/nursrep15060196.
The COVID-19 pandemic, caused by SARS-CoV-2, required the rapid development of diagnostic tests. SARS-CoV-2, part of the betacoronavirus genus, shares characteristics with SARS-CoV-1, including its ability to survive on surfaces, facilitating the spread of the infection. This study analyzes the technique of nasopharyngeal secretion collection for SARS-CoV-2 diagnosis and compares the accuracy of rapid antigen and molecular tests. : This study had two components: study A assessed the healthcare personnel training in collecting nasopharyngeal secretions and the discomfort associated with applying a questionnaire. Study B compared rapid antigen test accuracy with RT-PCR among children, through a retrospective analysis. The data were statistically analyzed to assess compliance with the testing protocols. : In study A, 88 healthcare workers achieved an average compliance score of 7.60 out of 10 regarding the collection procedure. Over 70% of participants correctly followed the fundamental steps of the procedure. Many patients who underwent sample collection reported pain and symptoms such as coughing or sneezing. In study B, 198 pediatric patients were tested using rapid antigen tests, collected simultaneously with RT-PCR. The rapid tests showed a 50% sensitivity and 97.5% specificity. : This study indicates that nasopharyngeal specimen collection techniques are based on international recommendations, but improvements could be made to reduce discomfort. Rapid antigen tests are helpful for screening due to their high specificity and negative predictive value. Continuous healthcare personnel training and the monitoring of diagnostic techniques remain essential in managing SARS-CoV-2 and other viral infections.
由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的2019冠状病毒病大流行，要求快速开发诊断检测方法。SARS-CoV-2属于β冠状病毒属，与严重急性呼吸综合征冠状病毒1（SARS-CoV-1）具有共同特征，包括其在物体表面存活的能力，这有利于感染传播。本研究分析了用于SARS-CoV-2诊断的鼻咽分泌物采集技术，并比较了快速抗原检测和分子检测的准确性。：本研究有两个部分：研究A评估了医护人员在采集鼻咽分泌物方面的培训情况以及应用问卷所涉及的不适情况。研究B通过回顾性分析比较了儿童中快速抗原检测与逆转录聚合酶链反应（RT-PCR）的准确性。对数据进行了统计分析以评估对检测方案的依从性。：在研究A中，88名医护人员在采集程序方面的平均依从性得分为7.60（满分10分）。超过70%的参与者正确遵循了该程序的基本步骤。许多接受样本采集的患者报告了疼痛以及咳嗽或打喷嚏等症状。在研究B中，198名儿科患者接受了快速抗原检测，同时采集了RT-PCR样本。快速检测的灵敏度为50%，特异性为97.5%。：本研究表明，鼻咽标本采集技术基于国际建议，但可以进行改进以减少不适。快速抗原检测因其高特异性和阴性预测值而有助于筛查。持续的医护人员培训以及诊断技术的监测在管理SARS-CoV-2和其他病毒感染方面仍然至关重要。
