Zhang Gang, Wang Xunming, Zhang Xingwang, Ye Lin, Ke Longyang, Fan Shimin, Hong Xuan, Li Guoqiang, Yang Bingye, Luo Lianzhong
Engineering Research Center of Marine Biopharmaceutical Resource of Fujian Province University, Xiamen Medical College, Xiamen 361023, China.
Xiamen Key Laboratory of Marine Medicinal Natural Product Resources, Xiamen Medical College, Xiamen 361023, China.
Mar Drugs. 2025 May 30;23(6):241. doi: 10.3390/md23060241.
is a common Gram-negative and facultative anaerobic bacillus that produces serratiochelins with several bioactivities. In this study, four catechol siderphores (-), including two new ones named serratiochelins E () and F (), were obtained from the fermentation of a mangrove-derived bacterium, . F2-2. The structures were elucidated with various spectroscopic methods such as NMR and HR-ESI-MS. Absolute and geometric configurations of the new compounds were established by employing quantum NMR calculations in conjunction with DP4+ probability analysis, ECD calculations, and the advanced Marfey's method. The bioactivity test showed that serratiochelin B () displayed weak but selective cytotoxicity against HepG2 cancer cells with an IC of 50.6 μmol/L and could trigger apoptosis through both Bcl-2/Bax/caspase-3 and Fas/FasL/caspase-8 signaling pathways. These findings deepen the understanding of siderophores of and provide a lead for research on anti-liver cancer drugs.
是一种常见的革兰氏阴性兼性厌氧杆菌，可产生具有多种生物活性的 Serratiochelins。在本研究中，从一株源自红树林的细菌F2-2的发酵产物中获得了四种儿茶酚铁载体（-），其中包括两种名为 Serratiochelins E（）和 F（）的新化合物。通过 NMR 和 HR-ESI-MS 等各种光谱方法阐明了其结构。利用量子 NMR 计算结合 DP4+概率分析、ECD 计算和先进的 Marfey 方法确定了新化合物的绝对构型和几何构型。生物活性测试表明，Serratiochelin B（）对 HepG2 癌细胞显示出微弱但具有选择性的细胞毒性，IC50为 50.6 μmol/L，并且可以通过 Bcl-2/Bax/caspase-3 和 Fas/FasL/caspase-8 信号通路触发细胞凋亡。这些发现加深了对该菌铁载体的理解，并为抗肝癌药物的研究提供了线索。
