Troia Roberta, Iannucci Claudia, Niemann Lisa, Vigani Alessio
Section of Small Animal Emergency and Critical Care, Department of Small Animals, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland.
Vet Sci. 2025 May 29;12(6):528. doi: 10.3390/vetsci12060528.
Double-filtration plasmapheresis (DFPP) is a plasma-exchange modality that allows selective clearance of high-molecular-weight proteins, potentially minimizing albumin loss and the need for volume replacement. Reports concerning DFPP use in dogs are scarce. This study evaluates the quantitative net loss of different plasma proteins fractions in an ex vivo model using canine blood processed via DFPP. DFPP treatment with INUSpheresis processing 1.5, 2, and 3 plasma volumes (PVs) was performed. Plasma proteins fractions were measured in the reservoir blood bag at baseline (pre-treatment) and in the effluent bag at the end of each target PV exchanged to calculate the net loss of selected plasma proteins. At 1.5 PV, net globulin and albumin loss was 41 and 25% respectively. At 3 PV, net globulin and albumin loss was 47 and 40%, respectively. Fibrinogen concentration was unmeasurable low in the reservoir blood bag after processing 1.5 PV. INUSpheresis allows selective plasma proteins removal, with a sparing effect on albumin at 1.5 PV. Selectivity is, however, progressively reduced with incremental target PV. A description of five DFPP treatments in three dogs is additionally presented. Semi-selective protein removal was also demonstrated in vivo, with a significantly lower percentage reduction in albumin compared to total globulin ( = 0.01) and fibrinogen ( = 0.007).
双重过滤血浆置换术（DFPP）是一种血浆置换方式，可选择性清除高分子量蛋白质，可能会将白蛋白损失和容量置换需求降至最低。关于DFPP在犬类中的应用报道较少。本研究在一个体外模型中，使用通过DFPP处理的犬类血液，评估不同血浆蛋白组分的定量净损失。采用INUSpheresis处理1.5、2和3个血浆量（PV）进行DFPP治疗。在基线（治疗前）时测量储血袋中的血浆蛋白组分，并在每个目标PV置换结束时测量流出袋中的血浆蛋白组分，以计算选定血浆蛋白的净损失。在1.5 PV时，球蛋白和白蛋白的净损失分别为41%和25%。在3 PV时，球蛋白和白蛋白的净损失分别为47%和40%。在处理1.5 PV后，储血袋中的纤维蛋白原浓度低至无法测量。INUSpheresis可选择性去除血浆蛋白，在1.5 PV时对白蛋白有保留作用。然而，随着目标PV的增加，选择性逐渐降低。此外还介绍了对三只犬进行五次DFPP治疗的情况。体内也证明了半选择性蛋白质清除，与总球蛋白（P = 0.01）和纤维蛋白原（P = 0.007）相比，白蛋白的降低百分比显著更低。
