Anoyatbekova Afshona, Komina Alina, Vlasova Natalia, Kononova Ekaterina, Gulyukin Alexey, Krasnikov Nikita, Yuzhakov Anton
Federal State Budget Scientific Institution "Federal Scientific Center VIEV", Moscow 109428, Russia.
Vet Sci. 2025 Jun 1;12(6):535. doi: 10.3390/vetsci12060535.
Porcine parvovirus 5 (PPV5) is a novel virus frequently detected in pigs worldwide. However, its identification and prevalence in domestic pigs in Russia have not been described. In this study, retrospectively, 984 serum samples collected between 2020 and 2023 were tested by qPCR. All samples were obtained from 20 pig farms located in 10 regions of Russia. PPV5 circulation was confirmed in 11 pig farms with the overall detection rate at 8.9%. The highest detection rate was estimated in the farms of the Belgorod Region (17.1%), followed by the Sverdlovsk Region (15.9%), the Republic of Buryatia (14.7%), and the Moscow Region (14.0%). It has been confirmed that PPV5 has been circulating in the swine population since at least 2021. The phylogenetic analysis revealed a high nucleotide identity of Russian isolates with strains from the USA, Colombia, and China. The Moscow-4060 strain was isolated in primary porcine testicular cells (PPTCs), and its continuous replication was confirmed by qPCR. During microscopy, cytoplasmic vacuolization, pyknosis, and other alterations were observed. The results demonstrate the evidence of PPV5 circulation in domestic pigs in Russia and the first isolation of the virus ever described. These findings serve as a first step for studying PPV5 in vivo.
猪细小病毒5型（PPV5）是一种新型病毒，在全球范围内的猪群中经常被检测到。然而，其在俄罗斯家猪中的鉴定和流行情况尚未见报道。在本研究中，回顾性地对2020年至2023年间收集的984份血清样本进行了qPCR检测。所有样本均来自俄罗斯10个地区的20个养猪场。在11个养猪场中证实了PPV5的传播，总体检出率为8.9%。别尔哥罗德州养猪场的检出率最高（17.1%），其次是斯维尔德洛夫斯克州（15.9%）、布里亚特共和国（14.7%）和莫斯科州（14.0%）。已证实PPV5至少自2021年以来一直在猪群中传播。系统发育分析显示，俄罗斯分离株与来自美国、哥伦比亚和中国的毒株具有高度的核苷酸同一性。莫斯科-4060毒株在原代猪睾丸细胞（PPTCs）中分离得到，并通过qPCR证实了其持续复制。在显微镜检查过程中，观察到细胞质空泡化、核固缩和其他变化。结果证明了PPV5在俄罗斯家猪中的传播证据以及该病毒的首次分离报道。这些发现是体内研究PPV5的第一步。
